Title |
Anal Microbial Patterns and Oncogenic Human Papillomavirus in a Pilot Study of Nigerian Men Who Have Sex with Men at Risk for or Living with HIV
|
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Published in |
AIDS Research and Human Retroviruses, October 2018
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DOI | 10.1089/aid.2018.0158 |
Pubmed ID | |
Authors |
Rebecca G. Nowak, Søren M. Bentzen, Jacques Ravel, Trevor A. Crowell, Wuese Dauda, Bing Ma, Hongjie Liu, William A. Blattner, Stefan D. Baral, Man E. Charurat, Manhattan Charurat, Julie Ake, Sylvia Adebajo, Stefan Baral, Erik Billings, Trevor Crowell, George Eluwa, Abiola Fasina, Charlotte Gaydos, Sosthenes Ketende, Afoke Kokogho, Hongjie Liu, Jennifer Malia, Olumide Makanjuola, Nelson Michael, Nicaise Ndembi, Jean Njab, Rebecca Nowak, Oluwasolape Olawore, Zahra Parker, Sheila Peel, Habib Ramadhani, Merlin Robb, Cristina Rodriguez-Hart, Eric Sanders-Buell, Sodsai Tovanabutra |
Abstract |
To cluster anal microbiota and define microbial patterns associated with biological, clinical, and behavioral correlates among Nigerian men who have sex with men (MSM) living with or at risk for HIV. In this cross-sectional pilot study, the 15 most abundant 16S taxa in the anal microbiota of 113 MSM underwent unsupervised K-means clustering and z-score comparisons to define similarities and dissimilarities among 4 microbiota taxonomic profiles. Distributions of oncogenic HPV (HR-HPV), concurrent HIV, antiretroviral therapy, and other clinical and behavioral data were evaluated using Fisher's exact and Kruskal-Wallis tests to determine biological signatures of cluster membership. Prevotella, was consistently represented in each cluster, but the average composition ranged from 14-44%. Cluster 2 was enriched with a member of the Fusobacteria phylum, Sneathia (29%). More participants of cluster 2 were HIV-infected and taking ART (83%, 5/6), were virally suppressed (80%, 4/5), had HPV-16 (66.7%, 4/6), and reported no vaginal sex partners (83%, 5/6). HPV-35, a highly prevalent oncogenic HPV in Nigeria, was observed in all clusters except cluster 2 (0%, 0/6). Other covariates were similar across clusters (all p>0.05). K-means unsupervised clustering, a canonical pattern recognition method, generalized the microbial community composition and structure while accounting for among-sample variability. Further studies are needed to evaluate whether an anal microbial community enriched with members of the Fusobacteria phylum is associated with HIV-infected MSM who are virally suppressed and have a concurrent HPV-16. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 90 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 16 | 18% |
Unspecified | 10 | 11% |
Researcher | 10 | 11% |
Student > Bachelor | 9 | 10% |
Student > Ph. D. Student | 9 | 10% |
Other | 13 | 14% |
Unknown | 23 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 21 | 23% |
Unspecified | 10 | 11% |
Biochemistry, Genetics and Molecular Biology | 7 | 8% |
Immunology and Microbiology | 6 | 7% |
Nursing and Health Professions | 6 | 7% |
Other | 12 | 13% |
Unknown | 28 | 31% |