Title |
Autophagy Promotes Microglia Activation Through Beclin-1-Atg5 Pathway in Intracerebral Hemorrhage
|
---|---|
Published in |
Molecular Neurobiology, January 2016
|
DOI | 10.1007/s12035-015-9642-z |
Pubmed ID | |
Authors |
Bangqing Yuan, Hanchao Shen, Li Lin, Tonggang Su, Lina Zhong, Zhao Yang |
Abstract |
Previous study demonstrates that intracerebral hemorrhage (ICH) promotes microglia activation and inflammation. However, the exact mechanism of microglia activation induced by ICH is not clear. In this experiment, microglia autophagy was examined using electron microscopy, conversion of light chain 3(LC3), and monodansylcadaverine (MDC) staining to detect autophagic vacuoles. We found that ICH induced microglia autophagy and activation. The suppression of autophagy using either pharmacologic inhibitors (3-methyladenine, bafilomycin A1) or RNA interference in essential autophagy genes (BECN1 and ATG5) decreased the microglia activation and inflammation in ICH. Moreover, autophagy inhibitors reduced brain damage in ICH. In conclusion, these data indicate that ICH contributes to microglia autophagic activation through BECN1 and ATG5 and provide the therapeutical strategy for ICH. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 3 | 13% |
Student > Bachelor | 3 | 13% |
Student > Ph. D. Student | 3 | 13% |
Researcher | 3 | 13% |
Student > Master | 1 | 4% |
Other | 2 | 9% |
Unknown | 8 | 35% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 30% |
Medicine and Dentistry | 5 | 22% |
Neuroscience | 2 | 9% |
Arts and Humanities | 1 | 4% |
Biochemistry, Genetics and Molecular Biology | 1 | 4% |
Other | 0 | 0% |
Unknown | 7 | 30% |