Title |
The impact of heating and soaking on the in vitro enzymatic hydrolysis of protein varies in different species of tropical legumes
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Published in |
Food Chemistry, August 2015
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DOI | 10.1016/j.foodchem.2015.08.022 |
Pubmed ID | |
Authors |
Julieta Torres, Shane M. Rutherfurd, Luz S. Muñoz, Michael Peters, Carlos A. Montoya |
Abstract |
The effects of different thermal (raw, autoclaving or boiling for 5 and 20min) and soaking (with or without) treatments on the degree of hydrolysis (DH) of protein were investigated for selected legumes (Canavalia brasiliensis; Lablab purpureus; pink, red and white colour hulls Vigna unguiculata). Each legume preparation underwent in vitro simulated gastrointestinal tract digestion comprising either pepsin (120min) or pepsin/pancreatin (120/240min) digestion. The DH was determined based on the amount of free amino groups released. Autoclaving for 5min increased the pepsin/pancreatin DH for all the unsoaked and soaked legumes (+20% to 46% units) except Canavalia, while boiling for 5min only increased DH for two soaked legumes (+12% to 28% units). Extending boiling from 5 to 20min increased the DH for three soaked legumes (+5% to 29% units). In conclusion, autoclaving, in general, extensively increased the sequential pepsin/pancreatin DH, while boiling only increased it for selected legumes. |
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