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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Brazilian red propolis: phytochemical screening, antioxidant activity and effect against cancer cells
|
|---|---|
| Published in |
BMC Complementary Medicine and Therapies, October 2015
|
| DOI | 10.1186/s12906-015-0888-9 |
| Pubmed ID | |
| Authors |
Izabel Cristina Gomes de Mendonça, Isabel Cristina Celerino de Moraes Porto, Ticiano Gomes do Nascimento, Naiana Soares de Souza, José Marcos dos Santos Oliveira, Rodolfo Elleson dos Santos Arruda, Kristiana Cerqueira Mousinho, Aldenir Feitosa dos Santos, Irinaldo Diniz Basílio-Júnior, Abhishek Parolia, Francisco Stefânio Barreto |
| Abstract |
The implementation of new public healthcare models that stimulate the use of natural products from traditional medicine, as a so-called integrated medicine, refers to an approach that use best of both conventional medicine and traditional medicine. Propolis is a widely used natural product by different ancient cultures and known to exhibit biological activities beneficial for health. The large number of studies conducted with propolis had shown that its chemical composition differs as a function of the climate, plant diversity and bee species and plays an important role on its therapeutic properties. The aim of this study was to analyse the phytochemical profile of the ethanolic extract of red propolis (EEP) and its fractionation, antioxidant action of EEP and its fractions hexane, cloroform and ethyl acetate and cytotoxic activity of EEP on human tumour cell lines SF-295 (glioblastoma), OVCAR-8 (ovary) and HCT-116 (colon). EEP was obtained by maceration with absolute ethanol, then it was concentrated in rotaevaporator up to complete evaporation of the solvent. The crude extract was fractionated with hexane, ethyl acetate, chloroform and methanol and they were subjected to phytochemical screening and total phenolic compounds. Antioxidant activity of EEP and fractions was done by means of the 2,2-diphenyl-1-picryhydrazyl (DPPH) method. Biomarkers of red propolis were identified by LC-Orbitrap-FTMS. To assess cytotoxic activity of the extract, cells were exposed to EEP over 72 h. Cell viability was assessed by means of MTT assay. The percentage of cell growth inhibition (IC50) was analysed by means of non-linear regression, and the absorbance values of the various investigated concentrations were subjected to one-factor analysis of variance (ANOVA) followed by Tukey's or Tamhane's tests (α = 0.05). The results obtained using phytochemical screening and LC-Orbitrap-FTMS indicated the presence of phlobaphene tannins, catechins, chalcones, aurones, flavonones, flavonols, xanthones, pentacyclic triterpenoids and guttiferones in Brazilian red propolis. EEP and its hexane, chloroform and ethyl acetate fractions obtained by liquid-liquid partitioning exhibited satisfactory antioxidant percentages. EEP (IC50 < 34.27 μg/mL) exhibited high levels of cytotoxicity on all human tumour cell lines tested when compared to negative control. C-Orbitrap-FTMS was useful to establish the chemical profile of the red propolis. Brazilian red propolis has antioxidant properties and decreases substantially the percentage of cell survival of human tumour cells; thus, it has potential to serve as an anticancer drug. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 50% |
| Tanzania, United Republic of | 1 | 25% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 266 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 5 | 2% |
| Unknown | 261 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 46 | 17% |
| Student > Master | 34 | 13% |
| Student > Ph. D. Student | 22 | 8% |
| Researcher | 18 | 7% |
| Student > Doctoral Student | 18 | 7% |
| Other | 51 | 19% |
| Unknown | 77 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 38 | 14% |
| Pharmacology, Toxicology and Pharmaceutical Science | 28 | 11% |
| Medicine and Dentistry | 27 | 10% |
| Biochemistry, Genetics and Molecular Biology | 26 | 10% |
| Chemistry | 19 | 7% |
| Other | 34 | 13% |
| Unknown | 94 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 September 2017.
All research outputs
#7,405,626
of 23,493,900 outputs
Outputs from BMC Complementary Medicine and Therapies
#1,199
of 3,697 outputs
Outputs of similar age
#90,284
of 280,838 outputs
Outputs of similar age from BMC Complementary Medicine and Therapies
#25
of 82 outputs
Altmetric has tracked 23,493,900 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 3,697 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.0. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,838 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 82 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.