Title |
Validation of extraction methods for total RNA and miRNA from bovine blood prior to quantitative gene expression analyses
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Published in |
Biotechnology Techniques, September 2009
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DOI | 10.1007/s10529-009-0130-2 |
Pubmed ID | |
Authors |
Andrea Hammerle-Fickinger, Irmgard Riedmaier, Christiane Becker, Heinrich H. D. Meyer, Michael W. Pfaffl, Susanne E. Ulbrich |
Abstract |
The benefit and precision of blood diagnosis by quantitative real-time PCR (qPCR) is limited by sampling procedures and RNA extraction methods. We have compared five different RNA extraction protocols from bovine blood regarding RNA and miRNA yield, quality, and most reproducible data in the qRT-PCR with the lowest point of quantification. Convincing results in terms of highest quantity, quality, and best performance for mRNA qPCR were obtained by leukocyte extraction following blood lysis as well as extraction of PAXgene stabilized blood. The best microRNA qPCR results were obtained for samples extracted by the leukocyte extraction method. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 2 | 2% |
Spain | 2 | 2% |
Malaysia | 1 | <1% |
France | 1 | <1% |
Austria | 1 | <1% |
Ireland | 1 | <1% |
Brazil | 1 | <1% |
United States | 1 | <1% |
Unknown | 107 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 46 | 39% |
Student > Ph. D. Student | 21 | 18% |
Student > Doctoral Student | 10 | 9% |
Student > Master | 10 | 9% |
Student > Bachelor | 5 | 4% |
Other | 9 | 8% |
Unknown | 16 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 49 | 42% |
Biochemistry, Genetics and Molecular Biology | 15 | 13% |
Medicine and Dentistry | 12 | 10% |
Veterinary Science and Veterinary Medicine | 4 | 3% |
Immunology and Microbiology | 2 | 2% |
Other | 14 | 12% |
Unknown | 21 | 18% |