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Calcium Signaling Protocols

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Cover of 'Calcium Signaling Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Fluorescent Measurement of [Ca 2+ ] c : Basic Practical Considerations
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    Chapter 2 Measurement of [ca(2+)] (I) in whole cell suspensions using fura-2.
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    Chapter 3 Confocal microscopy: theory and applications for cellular signaling.
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    Chapter 4 Ratiometric Ca 2+ Measurements Using the FlexStation ® Scanning Fluorometer
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    Chapter 5 Measuring Ca 2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR ® )
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    Chapter 6 Ratiometric [Ca(2+)] (i) Measurements in Adherent Cell-Lines Using the NOVOstar Microplate Reader.
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    Chapter 7 Whole-cell patch-clamp recording of voltage-sensitive ca(2+) channel currents in single cells: heterologous expression systems and neurones.
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    Chapter 8 Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells
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    Chapter 9 Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [ 3 H]Inositol Labeling Protocols in Permeabilized Cells
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    Chapter 10 Single-Cell Imaging Techniques for the Real-Time Detection of IP(3) in Live Cells.
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    Chapter 11 Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay
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    Chapter 12 Measurement of [Ca 2+ ] i in Smooth Muscle Strips Using Front-Surface Fluorimetry
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    Chapter 13 Calcium Measurements from Whole Heart Using Rhod-2
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    Chapter 14 Measurement of Changes in Endothelial and Smooth Muscle Ca 2+ in Pressurized Arteries
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    Chapter 15 Single Cell and Subcellular Measurements of Intracellular Ca 2+ Concentration
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    Chapter 16 Simultaneous Analysis of Intracellular pH and Ca(2+) from Cell Populations.
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    Chapter 17 Measurements of Ca 2+ Concentration with Recombinant Targeted Luminescent Probes
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    Chapter 18 Chimeric g proteins in fluorimetric calcium assays: experience with opioid receptors.
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    Chapter 20 Measurement of Cytosolic-Free Ca 2+ in Plant Tissue
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    Chapter 21 Measurement of Ca(2+)-ATPase Activity (in PMCA and SERCA1).
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