Title |
Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning
|
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Published in |
Biotechnology Reports, March 2016
|
DOI | 10.1016/j.btre.2016.03.003 |
Pubmed ID | |
Authors |
Suryani Saallah, M. Nazli Naim, I. Wuled Lenggoro, Mohd Noriznan Mokhtar, Noor Fitrah Abu Bakar, Masao Gen |
Abstract |
Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (-9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. A CGTase/PVA film made up from the same mixture and treated the same way was used as a control experiment. The immobilised CGTase on nanofibres showed superior performance with nearly a 2.5 fold higher enzyme loading and 31% higher enzyme activity in comparison with the film. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Japan | 1 | 1% |
Unknown | 84 | 99% |
Demographic breakdown
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Student > Ph. D. Student | 16 | 19% |
Student > Master | 16 | 19% |
Student > Bachelor | 12 | 14% |
Researcher | 8 | 9% |
Lecturer > Senior Lecturer | 4 | 5% |
Other | 12 | 14% |
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Chemistry | 14 | 16% |
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Other | 9 | 11% |
Unknown | 22 | 26% |