Chapter 1 Building a Two-Photon Microscope Is Easy

Chapter 2 Using Multiphoton Imaging for Targeted Electrophysiological Recording and Live Cell Imaging of Fluorescently Labeled Neurons from Isolated Retinas

Chapter 3 Two-Photon Neurotransmitter Uncaging for the Study of Dendritic Integration

Chapter 4 Two-Photon Glutamate Uncaging to Study Structural and Functional Plasticity of Dendritic Spines

Chapter 5 Two-Photon Fluorescence Imaging of Visually Evoked Glutamate Release Using iGluSnFR in the Mouse Visual System

Chapter 6 Imaging Neuronal Signal Transduction Using Multiphoton FRET-FLIM

Chapter 7 Combining Multiphoton Excitation Microscopy with Fast Microiontophoresis to Investigate Neuronal Signaling

Chapter 8 Multiphoton Excitation Microscopy for the Reconstruction and Analysis of Single Neuron Morphology

Chapter 9 Serial Multiphoton Tomography and Analysis of Volumetric Images of the Mouse Brain

Chapter 10 Studying a Light Sensor with Light: Multiphoton Imaging in the Retina

Chapter 11 Multiphoton Ca2+ Imaging of Astrocytes with Genetically Encoded Indicators Delivered by a Viral Approach

Chapter 12 Two-Photon Imaging of Dendritic Calcium Dynamics In Vivo

Chapter 13 Voltage Imaging with ANNINE Dyes and Two-Photon Microscopy

Chapter 14 High-Speed, Random-Access Multiphoton Microscopy for Monitoring Synaptic and Neuronal Activity in 3D in Behaving Animals