@ATinyGreenCell Nothing beats SLICE in cost efficiency and simplicity. https://t.co/uH9Ab4pue0 It does work.
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SLiCE法の元文献が SLiCE: a novel bacterial cell extract-based DNA cloning method Yongwei Zhang , Uwe Werling, Winfried Edelmann Nucleic Acids Res. 2012 40: e55. https://t.co/D4NFwVLn7L DH10B株というRecA欠損株でもRed組換え酵素なしでクローニング可能であることを示した
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@BiochemistTomas That's a good choice. I have almost no experience with Gibson, but this works beautifully for us https://t.co/uH9Ab4pue0 . And it is essentially free.
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RT @gramnegativity: there are many references but this is a good place to start https://t.co/jpXroEcGMl