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Mendeley readers
| Title |
Detection of proteases from Sporosarcina aquimarina and Algoriphagus antarcticus isolated from Antarctic soil
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|---|---|
| Published in |
Anais da Academia Brasileira de Ciências, March 2015
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| DOI | 10.1590/0001-3765201520130519 |
| Pubmed ID | |
| Authors |
Anderson F Santos, Fabiano Pires, Hugo E Jesus, André L S Santos, Raquel Peixoto, Alexandre S Rosado, Claudia M D'Avila-Levy, Marta H Branquinha |
| Abstract |
Two psychrophilic bacterial samples were isolated from King George Island soil, in Antarctica. The phylogenetic analysis based on the 16S rRNA (rrs) gene led to the correlation with the closest related isolates as Sporosarcina aquimarina (99%) and Algoriphagus antarcticus (99%), with query coverage of 99% and 98%, respectively. The spent culture media from both isolates displayed proteolytic activities detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis containing gelatin as protein substrate. Under the employed conditions, S. aquimarina showed a 55 kDa protease with the best activity detected at pH 7.0 and at 27°C. A. antarcticus also showed a single extracellular protease, however its molecular mass was around 90kDa and its best activity was detected at pH 9.0 and at 37°C. The proteases from both isolates were inhibited by 1,10-phenanthroline and EDTA, two metalloprotease inhibitors. This is the first record of protease detection in both species, and our results may contribute to broaden the basic knowledge of proteases from the Antarctica environment and may help prospecting future biotechnological applications of these enzymes. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 30 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 8 | 27% |
| Researcher | 7 | 23% |
| Student > Bachelor | 4 | 13% |
| Student > Ph. D. Student | 2 | 7% |
| Student > Doctoral Student | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 7 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 30% |
| Biochemistry, Genetics and Molecular Biology | 4 | 13% |
| Chemistry | 4 | 13% |
| Environmental Science | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Other | 3 | 10% |
| Unknown | 8 | 27% |