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Detection of proteases from Sporosarcina aquimarina and Algoriphagus antarcticus isolated from Antarctic soil

Overview of attention for article published in Anais da Academia Brasileira de Ciências, March 2015
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Title
Detection of proteases from Sporosarcina aquimarina and Algoriphagus antarcticus isolated from Antarctic soil
Published in
Anais da Academia Brasileira de Ciências, March 2015
DOI 10.1590/0001-3765201520130519
Pubmed ID
Authors

Anderson F. Santos, Fabiano Pires, Hugo E. Jesus, André L.S. Santos, Raquel Peixoto, Alexandre S. Rosado, Claudia M. D'Avila-Levy, Marta H. Branquinha

Abstract

Two psychrophilic bacterial samples were isolated from King George Island soil, in Antarctica. The phylogenetic analysis based on the 16S rRNA (rrs) gene led to the correlation with the closest related isolates as Sporosarcina aquimarina (99%) and Algoriphagus antarcticus (99%), with query coverage of 99% and 98%, respectively. The spent culture media from both isolates displayed proteolytic activities detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis containing gelatin as protein substrate. Under the employed conditions, S. aquimarina showed a 55 kDa protease with the best activity detected at pH 7.0 and at 27°C. A. antarcticus also showed a single extracellular protease, however its molecular mass was around 90kDa and its best activity was detected at pH 9.0 and at 37°C. The proteases from both isolates were inhibited by 1,10-phenanthroline and EDTA, two metalloprotease inhibitors. This is the first record of protease detection in both species, and our results may contribute to broaden the basic knowledge of proteases from the Antarctica environment and may help prospecting future biotechnological applications of these enzymes.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 32%
Researcher 5 23%
Student > Bachelor 4 18%
Student > Ph. D. Student 1 5%
Student > Doctoral Student 1 5%
Other 1 5%
Unknown 3 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 41%
Biochemistry, Genetics and Molecular Biology 2 9%
Chemistry 2 9%
Environmental Science 1 5%
Immunology and Microbiology 1 5%
Other 3 14%
Unknown 4 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 March 2015.
All research outputs
#11,064,272
of 12,446,028 outputs
Outputs from Anais da Academia Brasileira de Ciências
#198
of 318 outputs
Outputs of similar age
#183,002
of 219,429 outputs
Outputs of similar age from Anais da Academia Brasileira de Ciências
#6
of 8 outputs
Altmetric has tracked 12,446,028 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 318 research outputs from this source. They receive a mean Attention Score of 2.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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