Title |
Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil
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Published in |
Revista da Sociedade Brasileira de Medicina Tropical, January 2015
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DOI | 10.1590/0037-8682-0076-2015 |
Pubmed ID | |
Authors |
Tauana de Sousa Ferreira, Thaís Tâmara Castro Minuzzi-Souza, Andrey José de Andrade, Thais Oliveira Coelho, Douglas de Almeida Rocha, Marcos Takashi Obara, Mariana Hecht, Nadjar Nitz, Rodrigo Gurgel-Gonçalves |
Abstract |
This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil. Two hundred and ten females of 13 sand fly species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing. PCR revealed trypanosomatid-positive samples from Nyssomyia whitmani and Evandromyia evandroi, which were negative by kDNA and ITS1 Leishmania-specific PCRs. DNA sequence analysis of D7 24Sα rRNA amplicons indicated the occurrence of Blastocrithidia sp. and Trypanosoma sp. in Nyssomyia whitmani and Evandromyia evandroi, respectively. Two trypanosomatid species other than Leishmania sp. were found to circulate in sand flies in Central Brazil. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 8 | 30% |
Student > Bachelor | 4 | 15% |
Other | 2 | 7% |
Unspecified | 2 | 7% |
Student > Ph. D. Student | 2 | 7% |
Other | 3 | 11% |
Unknown | 6 | 22% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 8 | 30% |
Veterinary Science and Veterinary Medicine | 4 | 15% |
Unspecified | 2 | 7% |
Medicine and Dentistry | 2 | 7% |
Immunology and Microbiology | 2 | 7% |
Other | 1 | 4% |
Unknown | 8 | 30% |