Title |
Evaluation of phenotypic tests to detect carbapenem-resistant Enterobacteriaceae in colonized patients hospitalized in intensive care units
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Published in |
Brazilian Journal of Infectious Diseases, May 2015
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DOI | 10.1016/j.bjid.2015.03.008 |
Pubmed ID | |
Authors |
Leandro Reus Rodrigues Perez, Diógenes Rodrigues, Cícero Gomes Dias |
Abstract |
In this study, we aimed to evaluate the performance of different phenotypic tests to detect carbapenem-resistant Enterobacteriaceae (CRE). Three different phenotypic methods were evaluated: (1) combined-disk test (CDT) of meropenem plus phenylboronic acid (PBA) or EDTA reading after 24h and 48h (CDT-24 with PBA or EDTA and CDT-48 with PBA or EDTA, respectively); (2) selective/chromogenic read after 24h (ChromID-24h) and after 48h (ChromID-48h); and (3) overnight selective enrichment broth containing 10μg ertapenem disk followed by culture on MacConkey agar (SEB). A positive result in at least one of the methods was submitted to PCR for blaNDM-1, blaOXA-48, blaKPC, blaSPM-1, blaIMP, and blaGES detection. CRE was detected in 31 (30.4%) of 102 rectal swabs evaluated. All isolates showed to be KPC-2-producing organisms. Results showed excellent agreement among the evaluated tests (positive and negative) (kappa=0.88). It is important to state that CDT test with PBA is not suitable for bacterial identification/isolation. Conversely, ChromID-48h showed to be a useful tool, with the advantage of presumptive bacterial identification. |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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United States | 1 | 3% |
Unknown | 33 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 8 | 24% |
Student > Bachelor | 6 | 18% |
Researcher | 4 | 12% |
Student > Doctoral Student | 3 | 9% |
Student > Ph. D. Student | 2 | 6% |
Other | 5 | 15% |
Unknown | 6 | 18% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 6 | 18% |
Immunology and Microbiology | 6 | 18% |
Biochemistry, Genetics and Molecular Biology | 4 | 12% |
Nursing and Health Professions | 2 | 6% |
Other | 2 | 6% |
Unknown | 7 | 21% |