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Evaluation of phenotypic tests to detect carbapenem-resistant Enterobacteriaceae in colonized patients hospitalized in intensive care units

Overview of attention for article published in Brazilian Journal of Infectious Diseases, May 2015
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Title
Evaluation of phenotypic tests to detect carbapenem-resistant Enterobacteriaceae in colonized patients hospitalized in intensive care units
Published in
Brazilian Journal of Infectious Diseases, May 2015
DOI 10.1016/j.bjid.2015.03.008
Pubmed ID
Authors

Leandro Reus Rodrigues Perez, Diógenes Rodrigues, Cícero Gomes Dias

Abstract

In this study, we aimed to evaluate the performance of different phenotypic tests to detect carbapenem-resistant Enterobacteriaceae (CRE). Three different phenotypic methods were evaluated: (1) combined-disk test (CDT) of meropenem plus phenylboronic acid (PBA) or EDTA reading after 24h and 48h (CDT-24 with PBA or EDTA and CDT-48 with PBA or EDTA, respectively); (2) selective/chromogenic read after 24h (ChromID-24h) and after 48h (ChromID-48h); and (3) overnight selective enrichment broth containing 10μg ertapenem disk followed by culture on MacConkey agar (SEB). A positive result in at least one of the methods was submitted to PCR for blaNDM-1, blaOXA-48, blaKPC, blaSPM-1, blaIMP, and blaGES detection. CRE was detected in 31 (30.4%) of 102 rectal swabs evaluated. All isolates showed to be KPC-2-producing organisms. Results showed excellent agreement among the evaluated tests (positive and negative) (kappa=0.88). It is important to state that CDT test with PBA is not suitable for bacterial identification/isolation. Conversely, ChromID-48h showed to be a useful tool, with the advantage of presumptive bacterial identification.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 3%
Unknown 34 97%

Demographic breakdown

Readers by professional status Count As %
Student > Master 9 26%
Student > Bachelor 6 17%
Researcher 4 11%
Student > Doctoral Student 3 9%
Student > Ph. D. Student 2 6%
Other 5 14%
Unknown 6 17%
Readers by discipline Count As %
Medicine and Dentistry 7 20%
Immunology and Microbiology 7 20%
Agricultural and Biological Sciences 6 17%
Biochemistry, Genetics and Molecular Biology 4 11%
Nursing and Health Professions 2 6%
Other 2 6%
Unknown 7 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 June 2015.
All research outputs
#20,656,820
of 25,374,917 outputs
Outputs from Brazilian Journal of Infectious Diseases
#543
of 809 outputs
Outputs of similar age
#205,552
of 279,405 outputs
Outputs of similar age from Brazilian Journal of Infectious Diseases
#12
of 20 outputs
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