Title |
Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil
|
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Published in |
Revista Brasileira de Parasitologia Veterinária, June 2015
|
DOI | 10.1590/s1984-29612015037 |
Pubmed ID | |
Authors |
Cláudio Mafra, Cynthia Mantovani, Juliana Novo Borges, Rafael Mazioli Barcelos, Cláudia Portes Santos |
Abstract |
An anisakid nematode larva found in cod sold in the state of Minas Gerais, Brazil was studied by light and scanning electron microscopy and by a molecular approach. Mitochondrial cytochrome c-oxidase subunit 2 (mtDNA cox-2), 28S rRNA and ITS1, 5.8S and ITS2 regions were amplified using the polymerase chain reaction and sequenced to evaluate the phylogenetic relationships of the larva. The genetic profile confirmed that this larva belongs to the species Pseudoterranova decipiens (sensu stricto). This is the first molecular and ultrastructural study of Pseudoterranova decipiens (sensu stricto) in imported cod sold in Brazil. The health implications of these findings are discussed. |
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Mendeley readers
Geographical breakdown
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