Title |
Gallic acid conjugated with gold nanoparticles: antibacterial activity and mechanism of action on foodborne pathogens
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Published in |
International Journal of Nanomedicine, July 2016
|
DOI | 10.2147/ijn.s109795 |
Pubmed ID | |
Authors |
Narintorn Rattanata, Sompong Klaynongsruang, Chanvit Leelayuwat, Temduang Limpaiboon, Aroonlug Lulitanond, Patcharee Boonsiri, Sirinart Chio-Srichan, Siriwat Soontaranon, Supagorn Rugmai, Jureerut Daduang |
Abstract |
Foodborne pathogens, including Plesiomonas shigelloides and Shigella flexneri B, are the major cause of diarrheal endemics worldwide. Antibiotic drug resistance is increasing. Therefore, bioactive compounds with antibacterial activity, such as gallic acid (GA), are needed. Gold nanoparticles (AuNPs) are used as drug delivery agents. This study aimed to conjugate and characterize AuNP-GA and to evaluate the antibacterial activity. AuNP was conjugated with GA, and the core-shell structures were characterized by small-angle X-ray scattering and transmission electron microscopy. Antibacterial activity of AuNP-GA against P. shigelloides and S. flexneri B was evaluated by well diffusion method. AuNP-GA bactericidal mechanism was elucidated by Fourier transform infrared microspectroscopic analysis. The results of small-angle X-ray scattering showed that AuNP-GA conjugation was successful. Antibacterial activity of GA against both bacteria was improved by conjugation with AuNP because the minimum inhibitory concentration value of AuNP-GA was significantly decreased (P<0.0001) compared to that of GA. Fourier transform infrared analysis revealed that AuNP-GA resulted in alterations of lipids, proteins, and nucleic acids at the bacterial cell membrane. Our findings show that AuNP-GA has potential for further application in biomedical sciences. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 25% |
Unknown | 3 | 75% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 3 | 75% |
Members of the public | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 78 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 9 | 12% |
Student > Master | 8 | 10% |
Researcher | 7 | 9% |
Student > Bachelor | 7 | 9% |
Student > Doctoral Student | 3 | 4% |
Other | 11 | 14% |
Unknown | 33 | 42% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 12 | 15% |
Agricultural and Biological Sciences | 6 | 8% |
Chemistry | 6 | 8% |
Materials Science | 4 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 4% |
Other | 11 | 14% |
Unknown | 36 | 46% |