Title |
Unopposed Estrogen Supplementation/Progesterone Deficiency in Post‐Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue‐Specific Manner in the Rat
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Published in |
American Journal of Reproductive Immunology, August 2015
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DOI | 10.1111/aji.12424 |
Pubmed ID | |
Authors |
Stanislava Stanojević, Vesna Kovačević‐Jovanović, Mirjana Dimitrijević, Vesna Vujić, Ivana Ćuruvija, Veljko Blagojević, Gordana Leposavić |
Abstract |
The influence of unopposed estrogen replacement/isolated progesterone deficiency on macrophage production of pro-inflammatory/anti-inflammatory mediators in the post-reproductive age was studied. Considering that in the rats post-ovariectomy the circulating estradiol, but not progesterone level rises to the values in sham-operated controls, 20-month-old rats ovariectomized at the age of 10 months served as an experimental model. Estrogen and progesterone receptor expression, secretion of pro- and anti-inflammatory cytokines, and arginine metabolism end-products were examined in splenic and peritoneal macrophages under basal conditions and following lipopolysaccharide (LPS) stimulation in vitro. Almost all peritoneal and a subset of splenic macrophages expressed the intracellular progesterone receptor. Ovariectomy diminished cytokine production by splenic (IL-1β) and peritoneal (TNF-α, IL-1β, IL-10) macrophages and increased the production of IL-10 by splenic and TGF-β by peritoneal cells under basal conditions. Following LPS stimulation, splenic macrophages from ovariectomized rats produced less TNF-α and more IL-10, whereas peritoneal macrophages produced less IL-1β and TGF-β than the corresponding cells from sham-operated rats. Ovariectomy diminished urea production in both subpopulations of LPS-stimulated macrophages. Although long-lasting isolated progesterone deficiency in the post-reproductive age differentially affects cytokine production in the macrophages from distinct tissue compartments, in both subpopulations, it impairs the pro-inflammatory/anti-inflammatory cytokine secretory balance. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Other | 2 | 22% |
Professor | 1 | 11% |
Researcher | 1 | 11% |
Student > Ph. D. Student | 1 | 11% |
Unknown | 4 | 44% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 2 | 22% |
Agricultural and Biological Sciences | 1 | 11% |
Chemistry | 1 | 11% |
Immunology and Microbiology | 1 | 11% |
Unknown | 4 | 44% |