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Carrageenan delays cell cycle progression in human cancer cells in vitro demonstrated by FUCCI imaging

Overview of attention for article published in BMC Complementary and Alternative Medicine, August 2016
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2 tweeters

Citations

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87 Mendeley
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Title
Carrageenan delays cell cycle progression in human cancer cells in vitro demonstrated by FUCCI imaging
Published in
BMC Complementary and Alternative Medicine, August 2016
DOI 10.1186/s12906-016-1199-5
Pubmed ID
Authors

Eka Sunarwidhi Prasedya, Masao Miyake, Daisuke Kobayashi, Akihiro Hazama

Abstract

Carrageenan is a sulfated polysaccharide that exists in red seaweeds recently shown to have anticancer properties. Previous findings show various effects of carrageenan suppressing tumor cell growth. One of the hallmarks of cancer is uncontrolled proliferation, a consequence of loss of normal cell-cycle control, that underlies tumor growth. Recently there is an increasing interest in potential anticancer agents that affect cell cycle in cancer cells. Thus, in this study we investigated the effects of carrageenan on the tumor cell cycle. Using human cervical carcinoma cells (HeLa) cells as and human umbilical vein endothelial cells (HUVEC), the cytotoxic effects of kappa carrageenan (k-CO) and lambda carrageenan (λ-CO) at the concentrations of 250-2500 μg/mL were observed. Cell viability was determined using the MTT assay while cell death rates were determined using staining with calcein-AM/propidium iodide. Cell-cycle profile and progression were demonstrated with HeLa cells expressing FUCCI (fluorescence ubiquitination-based cell-cycle indicator) probes (HeLa-FUCCI). Carrageenan had no significant effect on HUVEC (normal cells). In contrast both forms of carrageenan were cytotoxic towards HeLa cells (cancer cells). Furthermore, according to cell-cycle analysis with FUCCI cells, the cell cycle of HeLa cells was delayed in specific phases due to different carrageenan treatments. Considering these results, it could be suggested that carrageenan affects the cell-cycle of HeLa cells not only by arresting the cell cycle in specific phases but also by delaying the time needed for the cell to progress through the cell cycle. Additionally, different types of carrageenans have different effects on cell cycle progression. This effect of carrageenan towards cancer cells could possibly be developed into a tumor cell-specific anticancer agent.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 87 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 87 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 11 13%
Lecturer 9 10%
Student > Ph. D. Student 8 9%
Student > Master 8 9%
Researcher 6 7%
Other 21 24%
Unknown 24 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 14 16%
Medicine and Dentistry 9 10%
Agricultural and Biological Sciences 8 9%
Pharmacology, Toxicology and Pharmaceutical Science 6 7%
Chemistry 6 7%
Other 10 11%
Unknown 34 39%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 July 2018.
All research outputs
#9,584,770
of 15,636,652 outputs
Outputs from BMC Complementary and Alternative Medicine
#1,433
of 2,967 outputs
Outputs of similar age
#139,793
of 267,108 outputs
Outputs of similar age from BMC Complementary and Alternative Medicine
#1
of 1 outputs
Altmetric has tracked 15,636,652 research outputs across all sources so far. This one is in the 36th percentile – i.e., 36% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,967 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.2. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,108 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them