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X Demographics
Mendeley readers
Attention Score in Context
| Title |
High Affinity Humanized Antibodies without Making Hybridomas; Immunization Paired with Mammalian Cell Display and In Vitro Somatic Hypermutation
|
|---|---|
| Published in |
PLOS ONE, November 2012
|
| DOI | 10.1371/journal.pone.0049458 |
| Pubmed ID | |
| Authors |
Audrey D. McConnell, Minjee Do, Tamlyn Y. Neben, Vladimir Spasojevic, Josh MacLaren, Andy P. Chen, Laurence Altobell, John L. Macomber, Ashley D. Berkebile, Robert A. Horlick, Peter M. Bowers, David J. King |
| Abstract |
A method has been developed for the rapid generation of high-affinity humanized antibodies from immunized animals without the need to make conventional hybridomas. Rearranged IgH D(J) regions were amplified from the spleen and lymph tissue of mice immunized with the human complement protein C5, fused with a limited repertoire of human germline heavy chain V-genes to form intact humanized heavy chains, and paired with a human light chain library. Completed heavy and light chains were assembled for mammalian cell surface display and transfected into HEK 293 cells co-expressing activation-induced cytidine deaminase (AID). Numerous clones were isolated by fluorescence-activated cell sorting, and affinity maturation, initiated by AID, resulted in the rapid evolution of high affinity, functional antibodies. This approach enables the efficient sampling of an immune repertoire and the direct selection and maturation of high-affinity, humanized IgGs. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Peru | 1 | 20% |
| Unknown | 4 | 80% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 80% |
| Science communicators (journalists, bloggers, editors) | 1 | 20% |
Mendeley readers
The data shown below were compiled from readership statistics for 93 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 2 | 2% |
| Portugal | 1 | 1% |
| Sweden | 1 | 1% |
| Mexico | 1 | 1% |
| United States | 1 | 1% |
| Unknown | 87 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 35 | 38% |
| Student > Ph. D. Student | 26 | 28% |
| Student > Master | 9 | 10% |
| Student > Bachelor | 5 | 5% |
| Other | 4 | 4% |
| Other | 9 | 10% |
| Unknown | 5 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 41 | 44% |
| Biochemistry, Genetics and Molecular Biology | 27 | 29% |
| Chemistry | 4 | 4% |
| Immunology and Microbiology | 3 | 3% |
| Engineering | 3 | 3% |
| Other | 9 | 10% |
| Unknown | 6 | 6% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 March 2023.
All research outputs
#2,710,551
of 24,387,992 outputs
Outputs from PLOS ONE
#33,945
of 210,272 outputs
Outputs of similar age
#18,705
of 182,633 outputs
Outputs of similar age from PLOS ONE
#631
of 4,734 outputs
Altmetric has tracked 24,387,992 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 210,272 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.6. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 182,633 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 4,734 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.