Title |
A Simplified Method for the Efficient Refolding and Purification of Recombinant Human GM-CSF
|
---|---|
Published in |
PLOS ONE, November 2012
|
DOI | 10.1371/journal.pone.0049891 |
Pubmed ID | |
Authors |
Christy A. Thomson, Melanie Olson, Linda M. Jackson, John W. Schrader |
Abstract |
Human granulocyte macrophage colony-stimulating factor (hGM-CSF) is a haematopoietic growth factor and proinflammatory cytokine. Recombinant hGM-CSF is important not only as a research tool but also as a biotherapeutic. However, rhGM-CSF expressed in E. coli is known to form inclusion bodies of misfolded, aggregated protein. Refolding and subsequent purification of rhGM-CSF from inclusion bodies is difficult with low yields of bioactive protein being produced. Here we describe a method for the isolation, refolding and purification of bioactive rhGM-CSF from inclusion bodies. The method is straightforward, not requiring extensive experience in protein refolding nor purification and using standard laboratory equipment. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Japan | 1 | <1% |
Poland | 1 | <1% |
Unknown | 139 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 33 | 23% |
Student > Ph. D. Student | 30 | 21% |
Student > Master | 21 | 15% |
Student > Bachelor | 14 | 10% |
Professor > Associate Professor | 8 | 6% |
Other | 11 | 8% |
Unknown | 24 | 17% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 46 | 33% |
Agricultural and Biological Sciences | 45 | 32% |
Chemistry | 9 | 6% |
Medicine and Dentistry | 4 | 3% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 2% |
Other | 7 | 5% |
Unknown | 27 | 19% |