Title |
DNA‐PK activity is associated with caspase‐dependent myogenic differentiation
|
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Published in |
FEBS Journal, October 2016
|
DOI | 10.1111/febs.13832 |
Pubmed ID | |
Authors |
Patrick F. Connolly, Howard O. Fearnhead |
Abstract |
Differentiation of myoblasts into myotubes is essential for skeletal muscle development and regeneration. Caspase-3 and caspase-9 are required for efficient myoblast differentiation. The caspase-activated endonuclease activity, CAD, and the DNA-damage repair protein XRCC1 have also been shown to be required to complete differentiation. DNA-damage associated with differentiation is accompanied by phosphorylation of Histone 2AX, an event normally catalysed by kinases ATR, ATM or DNA-PK. However, the kinase responsible for phosphorylation during differentiation is not known. Here we show that inhibition of DNA-PK, but not of ATR or ATM, blocked histone phosphorylation during differentiation. We also show that DNA-PK inhibition and siRNA-mediated DNA-PK knockdown blocked cell fusion. These data implicate a new role for DNA-PK in myogenic differentiation. This article is protected by copyright. All rights reserved. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 25% |
Other | 2 | 17% |
Student > Master | 2 | 17% |
Student > Bachelor | 1 | 8% |
Professor > Associate Professor | 1 | 8% |
Other | 0 | 0% |
Unknown | 3 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 58% |
Arts and Humanities | 1 | 8% |
Nursing and Health Professions | 1 | 8% |
Agricultural and Biological Sciences | 1 | 8% |
Unknown | 2 | 17% |