↓ Skip to main content

Label-Free Single-Particle Imaging of the Influenza Virus by Objective-Type Total Internal Reflection Dark-Field Microscopy

Overview of attention for article published in PLOS ONE, November 2012
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
3 X users
facebook
1 Facebook page

Readers on

mendeley
84 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Label-Free Single-Particle Imaging of the Influenza Virus by Objective-Type Total Internal Reflection Dark-Field Microscopy
Published in
PLOS ONE, November 2012
DOI 10.1371/journal.pone.0049208
Pubmed ID
Authors

Sawako Enoki, Ryota Iino, Nobuhiro Morone, Kunihiro Kaihatsu, Shouichi Sakakihara, Nobuo Kato, Hiroyuki Noji

Abstract

Here we report label-free optical imaging of single particles of the influenza virus attached on a glass surface with a simple objective-type total internal reflection dark-field microscopy (TIRDFM). The capability of TIRDFM for the imaging of single viral particles was confirmed from fine correlation of the TIRDFM images with fluorescent immunostaining image and scanning electron microscopy image. The density of scattering spots in the TIRDFM images showed a good linearity against the virus concentration, giving the limit of detection as 1.2×10(4) plaque-forming units per milliliter. Our label-free optical imaging method does require neither elaborated sample preparation nor complex optical systems, offering a good platform for rapid and sensitive counting of viral particles.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 84 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Denmark 1 1%
Italy 1 1%
Unknown 82 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 23 27%
Researcher 18 21%
Student > Bachelor 8 10%
Student > Master 5 6%
Student > Doctoral Student 3 4%
Other 10 12%
Unknown 17 20%
Readers by discipline Count As %
Chemistry 13 15%
Agricultural and Biological Sciences 13 15%
Physics and Astronomy 12 14%
Biochemistry, Genetics and Molecular Biology 10 12%
Engineering 7 8%
Other 8 10%
Unknown 21 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 November 2017.
All research outputs
#13,876,020
of 22,685,926 outputs
Outputs from PLOS ONE
#111,829
of 193,650 outputs
Outputs of similar age
#101,036
of 178,791 outputs
Outputs of similar age from PLOS ONE
#2,410
of 4,728 outputs
Altmetric has tracked 22,685,926 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 193,650 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.0. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 178,791 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 4,728 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.