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Development of a novel strategy for fungal transformation based on a mutant locus conferring carboxin-resistance in Magnaporthe oryzae

Overview of attention for article published in AMB Express, August 2016
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  • Above-average Attention Score compared to outputs of the same age and source (56th percentile)

Mentioned by

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1 tweeter
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1 Facebook page

Citations

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8 Dimensions

Readers on

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10 Mendeley
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Title
Development of a novel strategy for fungal transformation based on a mutant locus conferring carboxin-resistance in Magnaporthe oryzae
Published in
AMB Express, August 2016
DOI 10.1186/s13568-016-0232-x
Pubmed ID
Authors

Min Guo, Xiaolei Zhu, Hongxia Li, Leyong Tan, Yuemin Pan

Abstract

The accurate manipulation of genomic integration of mutant alleles or fluorescent fusion-protein constructs is necessary for understanding of pathogenic mechanism of Magnaporthe oryzae. Recently, this can be achieved by integrating of exogenous DNA randomly into genome of this pathogen, but ectopic integration may result in alteration of gene expression or gene disruption due to unpredictable position effects and/or disruption of protein-coding regions. In this study, we establish a novel strategy for locus-specific integration of exogenous DNA via carboxin-resistance reconstitution by a point-mutation (H245L) on succinate dehydrogenase subunit Mosdi1. Independent transformants derived from the same reconstitution construct showed consistent fluorescent signal and undiversified phenotypes, including hyphae growth, conidiation and pathogenicity, in M. oryzae. Meanwhile, 96 % of all transformants integrate correctly into the Mosdi1 locus as a single copy. Furthermore, we provide a vector carrying yeast recombination cassette and thus allow assembly of multiple overlapping DNA fragments by yeast in vivo recombination for gene complementation and protein localization assay.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 10%
Unknown 9 90%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 40%
Professor 2 20%
Student > Bachelor 2 20%
Researcher 1 10%
Librarian 1 10%
Other 0 0%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 70%
Biochemistry, Genetics and Molecular Biology 1 10%
Arts and Humanities 1 10%
Chemistry 1 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 August 2016.
All research outputs
#4,178,403
of 8,302,052 outputs
Outputs from AMB Express
#157
of 499 outputs
Outputs of similar age
#128,666
of 253,938 outputs
Outputs of similar age from AMB Express
#15
of 50 outputs
Altmetric has tracked 8,302,052 research outputs across all sources so far. This one is in the 47th percentile – i.e., 47% of other outputs scored the same or lower than it.
So far Altmetric has tracked 499 research outputs from this source. They receive a mean Attention Score of 2.1. This one has gotten more attention than average, scoring higher than 56% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 253,938 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 50 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.