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Adaptation to low pH and lignocellulosic inhibitors resulting in ethanolic fermentation and growth of Saccharomyces cerevisiae

Overview of attention for article published in AMB Express, August 2016
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Mentioned by

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2 tweeters

Citations

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24 Dimensions

Readers on

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71 Mendeley
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Title
Adaptation to low pH and lignocellulosic inhibitors resulting in ethanolic fermentation and growth of Saccharomyces cerevisiae
Published in
AMB Express, August 2016
DOI 10.1186/s13568-016-0234-8
Pubmed ID
Authors

Venkatachalam Narayanan, Violeta Sànchez i Nogué, Ed W. J. Niel, Marie F. Gorwa-Grauslund, Narayanan, Venkatachalam, Sànchez I Nogué, Violeta, van Niel, Ed W J, Gorwa-Grauslund, Marie F

Abstract

Lignocellulosic bioethanol from renewable feedstocks using Saccharomyces cerevisiae is a promising alternative to fossil fuels owing to environmental challenges. S. cerevisiae is frequently challenged by bacterial contamination and a combination of lignocellulosic inhibitors formed during the pre-treatment, in terms of growth, ethanol yield and productivity. We investigated the phenotypic robustness of a brewing yeast strain TMB3500 and its ability to adapt to low pH thereby preventing bacterial contamination along with lignocellulosic inhibitors by short-term adaptation and adaptive lab evolution (ALE). The short-term adaptation strategy was used to investigate the inherent ability of strain TMB3500 to activate a robust phenotype involving pre-culturing yeast cells in defined medium with lignocellulosic inhibitors at pH 5.0 until late exponential phase prior to inoculating them in defined media with the same inhibitor cocktail at pH 3.7. Adapted cells were able to grow aerobically, ferment anaerobically (glucose exhaustion by 19 ± 5 h to yield 0.45 ± 0.01 g ethanol g glucose(-1)) and portray significant detoxification of inhibitors at pH 3.7, when compared to non-adapted cells. ALE was performed to investigate whether a stable strain could be developed to grow and ferment at low pH with lignocellulosic inhibitors in a continuous suspension culture. Though a robust population was obtained after 3600 h with an ability to grow and ferment at pH 3.7 with inhibitors, inhibitor robustness was not stable as indicated by the characterisation of the evolved culture possibly due to phenotypic plasticity. With further research, this short-term adaptation and low pH strategy could be successfully applied in lignocellulosic ethanol plants to prevent bacterial contamination.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Sweden 1 1%
Unknown 70 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 27%
Researcher 13 18%
Student > Bachelor 12 17%
Student > Master 9 13%
Student > Postgraduate 3 4%
Other 7 10%
Unknown 8 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 25 35%
Biochemistry, Genetics and Molecular Biology 17 24%
Chemical Engineering 5 7%
Chemistry 3 4%
Engineering 3 4%
Other 8 11%
Unknown 10 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 August 2016.
All research outputs
#4,170,849
of 8,290,197 outputs
Outputs from AMB Express
#159
of 498 outputs
Outputs of similar age
#128,263
of 252,923 outputs
Outputs of similar age from AMB Express
#17
of 50 outputs
Altmetric has tracked 8,290,197 research outputs across all sources so far. This one is in the 47th percentile – i.e., 47% of other outputs scored the same or lower than it.
So far Altmetric has tracked 498 research outputs from this source. They receive a mean Attention Score of 2.0. This one has gotten more attention than average, scoring higher than 56% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 252,923 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 50 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.