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X Demographics
Mendeley readers
Attention Score in Context
| Title |
A Modular Cloning System for Standardized Assembly of Multigene Constructs
|
|---|---|
| Published in |
PLOS ONE, February 2011
|
| DOI | 10.1371/journal.pone.0016765 |
| Pubmed ID | |
| Authors |
Ernst Weber, Carola Engler, Ramona Gruetzner, Stefan Werner, Sylvestre Marillonnet |
| Abstract |
The field of synthetic biology promises to revolutionize biotechnology through the design of organisms with novel phenotypes useful for medicine, agriculture and industry. However, a limiting factor is the ability of current methods to assemble complex DNA molecules encoding multiple genetic elements in various predefined arrangements. We present here a hierarchical modular cloning system that allows the creation at will and with high efficiency of any eukaryotic multigene construct, starting from libraries of defined and validated basic modules containing regulatory and coding sequences. This system is based on the ability of type IIS restriction enzymes to assemble multiple DNA fragments in a defined linear order. We constructed a 33 kb DNA molecule containing 11 transcription units made from 44 individual basic modules in only three successive cloning steps. This modular cloning (MoClo) system can be readily automated and will be extremely useful for applications such as gene stacking and metabolic engineering. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 17% |
| United States | 1 | 17% |
| United Kingdom | 1 | 17% |
| Unknown | 3 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 4 | 67% |
| Members of the public | 2 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 2,160 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 14 | <1% |
| Germany | 12 | <1% |
| United Kingdom | 8 | <1% |
| Canada | 6 | <1% |
| France | 3 | <1% |
| Belgium | 3 | <1% |
| Israel | 1 | <1% |
| South Africa | 1 | <1% |
| Sweden | 1 | <1% |
| Other | 8 | <1% |
| Unknown | 2103 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 457 | 21% |
| Researcher | 350 | 16% |
| Student > Bachelor | 326 | 15% |
| Student > Master | 302 | 14% |
| Student > Doctoral Student | 73 | 3% |
| Other | 188 | 9% |
| Unknown | 464 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 761 | 35% |
| Biochemistry, Genetics and Molecular Biology | 689 | 32% |
| Engineering | 66 | 3% |
| Chemistry | 33 | 2% |
| Immunology and Microbiology | 20 | <1% |
| Other | 102 | 5% |
| Unknown | 489 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 49. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 May 2024.
All research outputs
#802,567
of 24,285,692 outputs
Outputs from PLOS ONE
#10,830
of 209,185 outputs
Outputs of similar age
#2,747
of 109,986 outputs
Outputs of similar age from PLOS ONE
#70
of 1,328 outputs
Altmetric has tracked 24,285,692 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 209,185 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.6. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 109,986 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 1,328 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.