Title |
Clinical identification of bacteria in human chronic wound infections: culturing vs. 16S ribosomal DNA sequencing
|
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Published in |
BMC Infectious Diseases, November 2012
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DOI | 10.1186/1471-2334-12-321 |
Pubmed ID | |
Authors |
Daniel D Rhoads, Stephen B Cox, Eric J Rees, Yan Sun, Randall D Wolcott |
Abstract |
Chronic wounds affect millions of people and cost billions of dollars in the United States each year. These wounds harbor polymicrobial biofilm communities, which can be difficult to elucidate using culturing methods. Clinical molecular microbiological methods are increasingly being employed to investigate the microbiota of chronic infections, including wounds, as part of standard patient care. However, molecular testing is more sensitive than culturing, which results in markedly different results being reported to clinicians. This study compares the results of aerobic culturing and molecular testing (culture-free 16S ribosomal DNA sequencing), and it examines the relative abundance score that is generated by the molecular test and the usefulness of the relative abundance score in predicting the likelihood that the same organism would be detected by culture. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 2 | 1% |
Portugal | 1 | <1% |
Canada | 1 | <1% |
Spain | 1 | <1% |
Poland | 1 | <1% |
Unknown | 192 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 32 | 16% |
Student > Bachelor | 29 | 15% |
Student > Master | 26 | 13% |
Researcher | 25 | 13% |
Other | 9 | 5% |
Other | 34 | 17% |
Unknown | 43 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 40 | 20% |
Immunology and Microbiology | 32 | 16% |
Biochemistry, Genetics and Molecular Biology | 23 | 12% |
Medicine and Dentistry | 21 | 11% |
Nursing and Health Professions | 7 | 4% |
Other | 22 | 11% |
Unknown | 53 | 27% |