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Mendeley readers
| Chapter title |
Quantitative Real-Time PCR Analysis of Gene Transcripts of Mosquito Follicles.
|
|---|---|
| Chapter number | 9 |
| Book title |
Oogenesis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3795-0_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3793-6, 978-1-4939-3795-0
|
| Authors |
Aparna Telang |
| Editors |
Ioannis P. Nezis |
| Abstract |
Real-time (quantitative) PCR, or QPCR, has become an indispensible tool for characterizing gene expression. Depending on the experimental design, researchers can use either the relative or absolute (standard curve) method to quantify transcript abundance. Characterizing the expression of genes in mosquito ovaries will require use of the standard curve method of quantification. Here, I describe reagents and equipment necessary to run standard curve QPCR. I also provide details on the construction of the standard linear curve and calculations required to determine transcript abundance. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 29% |
| Student > Master | 2 | 29% |
| Student > Postgraduate | 1 | 14% |
| Student > Ph. D. Student | 1 | 14% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 29% |
| Psychology | 1 | 14% |
| Sports and Recreations | 1 | 14% |
| Social Sciences | 1 | 14% |
| Unknown | 2 | 29% |