Chapter title |
Chemiluminescence assays to investigate membrane expression and clathrin-mediated endocytosis of K(ATP) channels.
|
---|---|
Chapter number | 4 |
Book title |
Potassium Channels
|
Published in |
Methods in molecular biology, January 2008
|
DOI | 10.1007/978-1-59745-526-8_4 |
Pubmed ID | |
Book ISBNs |
978-1-934115-65-7, 978-1-59745-526-8
|
Authors |
Smith, Andrew J, Sivaprasadarao, Asipu, Smith, Andrew J., Andrew J. Smith, Asipu Sivaprasadarao |
Abstract |
Macroscopic ion channel currents (I) are a product of the channel open probability (P (o)), the single channel current (i) and the number of channels present on the cell surface (N) at any given time (I = P (o) iN). Endocytosis has been shown to be one of the key determinants of cell surface channel density and the defects of this process have been linked to diseases relating to ion channel dysfunction. Chemiluminescence-based techniques provide a rapid method for the examination of the rates of endocytosis and steady-state cell surface density of ion channels and have previously been used to investigate the endocytosis of pancreatic ATP-sensitive potassium (K(ATP)) channels. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 18% |
Student > Ph. D. Student | 2 | 18% |
Other | 1 | 9% |
Student > Master | 1 | 9% |
Professor > Associate Professor | 1 | 9% |
Other | 0 | 0% |
Unknown | 4 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 3 | 27% |
Biochemistry, Genetics and Molecular Biology | 1 | 9% |
Agricultural and Biological Sciences | 1 | 9% |
Nursing and Health Professions | 1 | 9% |
Unknown | 5 | 45% |