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Mendeley readers
Attention Score in Context
| Title |
A Fast Universal Immobilization of Immunoglobulin G at 4°C for the Development of Array-based Immunoassays
|
|---|---|
| Published in |
PLOS ONE, December 2012
|
| DOI | 10.1371/journal.pone.0051370 |
| Pubmed ID | |
| Authors |
Shu-Lin Guo, Po-Chung Chen, Ming-Shuo Chen, Yu-Che Cheng, Jun-Mu Lin, Hoong-Chien Lee, Chien-Sheng Chen |
| Abstract |
To maintain the antibody activity and enhance performance of array-based immunoassays, protein G was used to allow a shorter duration of immunoglobulin G immobilization at 4 °C, with the antibody placed in the appropriate orientation. The multiplexed detection of six pain-related message molecules (PRMMs) was used as examples for the development of array-based immunoassays: substance P, calcitonin gene-related peptide, nerve growth factor, brain-derived neurotrophic factor, tumor necrosis factor-α, and β-endorphin. Protein G- and non-protein G-coated slides were tested. Compared to non-protein G immunoassays, protein G shortened the antibody immobilization time at 4 °C from overnight to 2 hours. Only protein G-facilitated immunoassays succeeded in simultaneously detecting all six PRMMs with high specificity. Dose-response curves showed that the limits of detection of the protein G-multiplexed immunoassays for the PRMMs was approximately 164, 167, 120, 60, 80, and 92 pg/ml, respectively. Thus, protein G effectively shortens the duration of antibody immobilization at 4 °C, allowing the use of sensitive array-based immunoassays for the simultaneous detection of PRMMs. |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 4% |
| Sweden | 1 | 4% |
| Unknown | 26 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 18% |
| Researcher | 5 | 18% |
| Student > Ph. D. Student | 4 | 14% |
| Student > Doctoral Student | 3 | 11% |
| Student > Bachelor | 3 | 11% |
| Other | 6 | 21% |
| Unknown | 2 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 18% |
| Agricultural and Biological Sciences | 5 | 18% |
| Engineering | 4 | 14% |
| Medicine and Dentistry | 4 | 14% |
| Physics and Astronomy | 2 | 7% |
| Other | 5 | 18% |
| Unknown | 3 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 December 2012.
All research outputs
#20,176,348
of 22,689,790 outputs
Outputs from PLOS ONE
#172,810
of 193,655 outputs
Outputs of similar age
#246,364
of 277,812 outputs
Outputs of similar age from PLOS ONE
#3,931
of 4,765 outputs
Altmetric has tracked 22,689,790 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 193,655 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 277,812 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 4,765 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.