| Chapter title |
Preparation of Proteoglycan Mimetic Graft Copolymers
|
|---|---|
| Chapter number | 7 |
| Book title |
Macro-Glycoligands
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3130-9_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3129-3, 978-1-4939-3130-9
|
| Authors |
Matt J. Kipper, Laura W. Place |
| Abstract |
Proteoglycans are proteins with pendant glycosaminoglycan polysaccharide side chains. The method described here enables the preparation of graft copolymers with glycosaminoglycan side chains, which mimic the structure and composition of proteoglycans. By controlling the stoichiometry, graft copolymers can be obtained with a wide range of glycosaminoglycan side-chain densities. The method presented here uses a three-step reaction mechanism to first functionalize a hyaluronic acid backbone, followed by reductive amination to couple the glycosaminoglycan side chain to the backbone, by the reducing end. Proteoglycan mimics like the ones proposed here could be used to study the structure-property relationships of proteoglycans and to introduce the biochemical and biomechanical properties of proteoglycans into biomaterials and therapeutic formulations. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 2 | 29% |
| Unspecified | 1 | 14% |
| Student > Ph. D. Student | 1 | 14% |
| Researcher | 1 | 14% |
| Unknown | 2 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemical Engineering | 2 | 29% |
| Unspecified | 1 | 14% |
| Chemistry | 1 | 14% |
| Engineering | 1 | 14% |
| Unknown | 2 | 29% |