↓ Skip to main content

Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies

Overview of attention for article published in PLOS ONE, January 2013
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (90th percentile)
  • High Attention Score compared to outputs of the same age and source (85th percentile)

Mentioned by

patent
3 patents
wikipedia
5 Wikipedia pages

Citations

dimensions_citation
65 Dimensions

Readers on

mendeley
67 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies
Published in
PLOS ONE, January 2013
DOI 10.1371/journal.pone.0053446
Pubmed ID
Authors

Emmanuel Bréard, Estelle Lara, Loïc Comtet, Cyril Viarouge, Virginie Doceul, Alexandra Desprat, Damien Vitour, Nathalie Pozzi, Ann Brigitte Cay, Nick De Regge, Philippe Pourquier, Horst Schirrmeier, Bernd Hoffmann, Martin Beer, Corinne Sailleau, Stéphan Zientara

Abstract

A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 were categorized according to the results of a virus neutralization test (VNT) or an indirect immuno-fluorescence assay (IFA). The specificity was evaluated with 1364 sera from sheep, goat and bovine collected in France and Belgium before 2009. Overall agreement between VNT and ELISA was 98.9% and 98.3% between VNT and IFA, indicating a very good concordance between the different techniques. Although cross-reactions with other Orthobunyavirus from the Simbu serogroup viruses might occur, it is a highly sensitive, specific and robust ELISA-test validated to detect anti-SBV antibodies. This test can be applied for SBV sero-diagnostics and disease-surveillance studies in ruminant species in Europe.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 1%
Unknown 66 99%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 16%
Student > Master 11 16%
Student > Ph. D. Student 10 15%
Student > Doctoral Student 9 13%
Student > Bachelor 5 7%
Other 11 16%
Unknown 10 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 18 27%
Veterinary Science and Veterinary Medicine 17 25%
Biochemistry, Genetics and Molecular Biology 9 13%
Immunology and Microbiology 3 4%
Medicine and Dentistry 3 4%
Other 7 10%
Unknown 10 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 December 2022.
All research outputs
#2,552,985
of 23,437,201 outputs
Outputs from PLOS ONE
#32,401
of 200,534 outputs
Outputs of similar age
#29,179
of 310,270 outputs
Outputs of similar age from PLOS ONE
#709
of 4,854 outputs
Altmetric has tracked 23,437,201 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 200,534 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.3. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 310,270 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 4,854 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.