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The Tumor Microenvironment

Overview of attention for book
Cover of 'The Tumor Microenvironment'

Table of Contents

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    Book Overview
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    Chapter 1 Methods of Immunohistochemistry and Immunofluorescence: Converting Invisible to Visible.
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    Chapter 2 Laser Capture Microdissection as a Tool to Study Tumor Stroma.
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    Chapter 3 Quantitative Analysis of Human Cancer Cell Extravasation Using Intravital Imaging.
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    Chapter 4 Studies on the Tumor Vasculature and Coagulant Microenvironment.
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    Chapter 5 A Microfluidic Method to Mimic Luminal Structures in the Tumor Microenvironment.
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    Chapter 6 Measuring Vascular Permeability In Vivo.
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    Chapter 7 The Tumor Microenvironment
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    Chapter 8 Analyzing the Tumor Microenvironment by Flow Cytometry.
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    Chapter 9 The Tumor Microenvironment
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    Chapter 10 Purification of Immune Cell Populations from Freshly Isolated Murine Tumors and Organs by Consecutive Magnetic Cell Sorting and Multi-parameter Flow Cytometry-Based Sorting.
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    Chapter 11 Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes.
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    Chapter 12 The Tumor Microenvironment
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    Chapter 13 Isolation and Characterization of Low- vs. High-Density Neutrophils in Cancer.
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    Chapter 14 Analysis of Extracellular Vesicles in the Tumor Microenvironment.
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    Chapter 15 Visualizing the Tumor Microenvironment of Liver Metastasis by Spinning Disk Confocal Microscopy.
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    Chapter 16 Intravital Microscopy for Imaging the Tumor Microenvironment in Live Mice.
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    Chapter 17 The Tumor Microenvironment
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    Chapter 18 The Tumor Microenvironment
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    Chapter 19 CRISPR/Cas9 Genome Editing as a Strategy to Study the Tumor Microenvironment in Transgenic Mice.
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    Chapter 20 The Tumor Microenvironment
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    Chapter 21 The Tumor Microenvironment
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    Chapter 22 RNA-Seq as a Tool to Study the Tumor Microenvironment.
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    Chapter 23 Sample Preparation for Mass Spectrometry Analysis of Protein-Protein Interactions in Cancer Cell Lines and Tissues.
Attention for Chapter 19: CRISPR/Cas9 Genome Editing as a Strategy to Study the Tumor Microenvironment in Transgenic Mice.
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Chapter title
CRISPR/Cas9 Genome Editing as a Strategy to Study the Tumor Microenvironment in Transgenic Mice.
Chapter number 19
Book title
The Tumor Microenvironment
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3801-8_19
Pubmed ID
27581028
Book ISBNs
978-1-4939-3799-8, 978-1-4939-3801-8
Authors

Yojiro Yamanaka

Editors

Josie Ursini-Siegel, Nicole Beauchemin

Abstract

Development of engineered site-specific endonucleases like zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9 has been revolutionizing genetic approaches in biomedical research fields. These new tools have opened opportunities to carry out targeted genome editing in mouse zygotes without the need for manipulating embryonic stem cells, which have a higher technical burden and many constraints in strain availability. Specific genetic modifications can be directly generated in working genetic backgrounds. This new approach saves time and costs associated with generation and backcrossing of genetically modified animals and will facilitate their use in various cancer research fields.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 38%
Student > Bachelor 1 13%
Student > Master 1 13%
Unknown 3 38%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 25%
Agricultural and Biological Sciences 2 25%
Immunology and Microbiology 2 25%
Social Sciences 1 13%
Unknown 1 13%

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