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The Wilms' Tumor (WT1) Gene

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Cover of 'The Wilms' Tumor (WT1) Gene'

Table of Contents

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    Book Overview
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    Chapter 1 WT1 Mutation in Childhood Cancer.
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    Chapter 2 Clinical Aspects of WT1 and the Kidney.
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    Chapter 3 The Role of WT1 in Embryonic Development and Normal Organ Homeostasis.
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    Chapter 4 Tools and Techniques for Wt1-Based Lineage Tracing.
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    Chapter 5 Biological Systems and Methods for Studying WT1 in the Epicardium.
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    Chapter 6 Isolation and Colony Formation of Murine Bone and Bone Marrow Cells.
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    Chapter 7 Isolation and Fluorescence-Activated Cell Sorting of Murine WT1-Expressing Adipocyte Precursor Cells.
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    Chapter 8 The Wilms' Tumor (WT1) Gene
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    Chapter 9 Multiphoton Microscopy for Visualizing Lipids in Tissue.
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    Chapter 10 Function and Regulation of the Wilms' Tumor Suppressor 1 (WT1) Gene in Fish.
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    Chapter 11 Immunofluorescence Staining of Wt1 on Sections of Zebrafish Embryos and Larvae.
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    Chapter 12 Fluorescence-Activated Cell Sorting (FACS) Protocol for Podocyte Isolation in Adult Zebrafish.
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    Chapter 13 In Vitro Transcription to Study WT1 Function.
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    Chapter 14 Measuring Equilibrium Binding Constants for the WT1-DNA Interaction Using a Filter Binding Assay.
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    Chapter 15 The Wilms' Tumor (WT1) Gene
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    Chapter 16 The Wilms' Tumor (WT1) Gene
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    Chapter 17 Methods to Identify and Validate WT1-RNA Interaction.
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    Chapter 18 The Wilms' Tumor (WT1) Gene
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    Chapter 19 The Wilms' Tumor (WT1) Gene
Attention for Chapter 16: The Wilms' Tumor (WT1) Gene
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Chapter title
The Wilms' Tumor (WT1) Gene
Chapter number 16
Book title
The Wilms' Tumor (WT1) Gene
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-4023-3_16
Pubmed ID
Book ISBNs
978-1-4939-4021-9, 978-1-4939-4023-3
Authors

Bharathavikru, Ruthrothaselvi, von Kriegsheim, Alex, Ruthrothaselvi Bharathavikru, Alex von Kriegsheim

Editors

Nicholas Hastie

Abstract

Tumor-suppressor protein Wt1 has been shown to interact with specific proteins that influence its function. These protein interactions have been identified as direct individual interactions but with the potential to exist as a part of a multiprotein complex. In order to obtain the global proteome interaction map of Wt1, an unbiased label-free endogenous immunoprecipitation was performed followed by mass spectrometry to identify protein interactions that are Wt1 centric. This chapter details the different techniques that have been used to identify and characterize Wt1-interacting proteins.

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Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 20%
Researcher 1 20%
Student > Master 1 20%
Unknown 2 40%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 40%
Immunology and Microbiology 1 20%
Unknown 2 40%