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Mendeley readers
Attention Score in Context
| Title |
Streamlining Homogeneous Glycoprotein Production for Biophysical and Structural Applications by Targeted Cell Line Development
|
|---|---|
| Published in |
PLOS ONE, December 2011
|
| DOI | 10.1371/journal.pone.0027829 |
| Pubmed ID | |
| Authors |
Sonja Wilke, Lothar Groebe, Vitali Maffenbeier, Volker Jäger, Manfred Gossen, Jörn Josewski, Agathe Duda, Lilia Polle, Raymond J. Owens, Dagmar Wirth, Dirk W. Heinz, Joop van den Heuvel, Konrad Büssow |
| Abstract |
Studying the biophysical characteristics of glycosylated proteins and solving their three-dimensional structures requires homogeneous recombinant protein of high quality.We introduce here a new approach to produce glycoproteins in homogenous form with the well-established, glycosylation mutant CHO Lec3.2.8.1 cells. Using preparative cell sorting, stable, high-expressing GFP 'master' cell lines were generated that can be converted fast and reliably by targeted integration via Flp recombinase-mediated cassette exchange (RMCE) to produce any glycoprotein. Small-scale transient transfection of HEK293 cells was used to identify genetically engineered constructs suitable for constructing stable cell lines. Stable cell lines expressing 10 different proteins were established. The system was validated by expression, purification, deglycosylation and crystallization of the heavily glycosylated luminal domains of lysosome-associated membrane proteins (LAMP). |
Mendeley readers
The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Germany | 1 | 2% |
| Unknown | 57 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 22 | 37% |
| Student > Ph. D. Student | 11 | 19% |
| Student > Master | 10 | 17% |
| Student > Bachelor | 3 | 5% |
| Student > Doctoral Student | 2 | 3% |
| Other | 5 | 8% |
| Unknown | 6 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 30 | 51% |
| Biochemistry, Genetics and Molecular Biology | 11 | 19% |
| Chemistry | 2 | 3% |
| Immunology and Microbiology | 2 | 3% |
| Environmental Science | 1 | 2% |
| Other | 4 | 7% |
| Unknown | 9 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 December 2022.
All research outputs
#3,220,155
of 23,377,816 outputs
Outputs from PLOS ONE
#42,421
of 199,897 outputs
Outputs of similar age
#25,870
of 243,919 outputs
Outputs of similar age from PLOS ONE
#457
of 2,931 outputs
Altmetric has tracked 23,377,816 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 199,897 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.3. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 243,919 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 2,931 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.