Title |
Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation
|
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Published in |
BMC Biology, January 2013
|
DOI | 10.1186/1741-7007-11-4 |
Pubmed ID | |
Authors |
Tyson A Clark, Xingyu Lu, Khai Luong, Qing Dai, Matthew Boitano, Stephen W Turner, Chuan He, Jonas Korlach |
Abstract |
DNA methylation serves as an important epigenetic mark in both eukaryotic and prokaryotic organisms. In eukaryotes, the most common epigenetic mark is 5-methylcytosine, whereas prokaryotes can have 6-methyladenine, 4-methylcytosine, or 5-methylcytosine. Single-molecule, real-time sequencing is capable of directly detecting all three types of modified bases. However, the kinetic signature of 5-methylcytosine is subtle, which presents a challenge for detection. We investigated whether conversion of 5-methylcytosine to 5-carboxylcytosine using the enzyme Tet1 would enhance the kinetic signature, thereby improving detection. |
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Mendeley readers
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