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Heterologous Expression of Membrane Proteins

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Cover of 'Heterologous Expression of Membrane Proteins'

Table of Contents

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    Book Overview
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    Chapter 1 Heterologous Expression of Membrane Proteins
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    Chapter 2 Heterologous Expression of Membrane Proteins
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    Chapter 3 Membrane Protein Production in Escherichia coli: Protocols and Rules.
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    Chapter 4 Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach.
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    Chapter 5 Generation of Tetracycline-Inducible Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane Proteins.
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    Chapter 6 Membrane Protein Production in Lactococcus lactis for Functional Studies.
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    Chapter 7 Heterologous Expression of Membrane Proteins
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    Chapter 8 Leishmania tarentolae as a Promising Tool for Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABC Pump ABCG6.
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    Chapter 9 Overexpression, Membrane Preparation, and Purification of a Typical Multidrug ABC Transporter BmrA.
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    Chapter 10 Heterologous Expression of Membrane Proteins
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    Chapter 11 Heterologous Expression of Membrane Proteins
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    Chapter 12 Heterologous Expression of Membrane Proteins
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    Chapter 13 Heterologous Expression of Membrane Proteins
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    Chapter 14 Heterologous Expression of Membrane Proteins
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    Chapter 15 Membrane Protein Solubilization and Composition of Protein Detergent Complexes.
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    Chapter 16 Detergent-Free Membrane Protein Purification.
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    Chapter 17 Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry.
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    Chapter 18 Lessons from an α-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization.
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    Chapter 19 Heterologous Expression of Membrane Proteins
Attention for Chapter 17: Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry.
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Chapter title
Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry.
Chapter number 17
Book title
Heterologous Expression of Membrane Proteins
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3637-3_17
Pubmed ID
Book ISBNs
978-1-4939-3635-9, 978-1-4939-3637-3
Authors

Eric Forest, Petr Man

Editors

Isabelle Mus-Veteau

Abstract

Hydrogen/deuterium exchange associated with mass spectrometry has been recently used to characterize the dynamics and the interactions of membrane proteins. Here we describe experimental workflow enabling localization of the regions involved in conformational changes or interactions.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Other 3 27%
Student > Ph. D. Student 2 18%
Student > Doctoral Student 1 9%
Unspecified 1 9%
Researcher 1 9%
Other 1 9%
Unknown 2 18%
Readers by discipline Count As %
Computer Science 3 27%
Neuroscience 2 18%
Agricultural and Biological Sciences 1 9%
Unspecified 1 9%
Biochemistry, Genetics and Molecular Biology 1 9%
Other 1 9%
Unknown 2 18%