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Clarification of the molecular pathway of Taiwan local pomegranate fruit juice underlying the inhibition of urinary bladder urothelial carcinoma cell by proteomics strategy

Overview of attention for article published in BMC Complementary and Alternative Medicine, March 2016
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Title
Clarification of the molecular pathway of Taiwan local pomegranate fruit juice underlying the inhibition of urinary bladder urothelial carcinoma cell by proteomics strategy
Published in
BMC Complementary and Alternative Medicine, March 2016
DOI 10.1186/s12906-016-1071-7
Pubmed ID
Authors

Ting-Feng Wu, Li-Ting Hsu, Bo-Xian Tsang, Li-Chien Huang, Wan-Yin Shih, Li-Yi Chen

Abstract

Pomegranate fruit has been shown to exhibit the inhibitory activity against prostate cancer and lung cancer in vitro and in vivo, which might be a resource for chemoprevention and chemotherapy of cancer. Our previous documented findings indicated that treatment of urinary bladder urothelial carcinoma cell with the ethanol extract isolated from the juice of pomegranate fruit grown in Taiwan could inhibit tumor cell. In this study we intended to uncover the molecular pathway underlying anti-cancer efficacy of Taiwan pomegranate fruit juice against urinary bladder urothelial carcinoma. We exploited two-dimensional gel electrophoresis coupled with tandem mass spectrometry to find the de-regulated proteins. Western immunoblotting was used to confirm the results collected from proteomics study. Comparative proteomics indicated that 20 proteins were differentially expressed in ethanol extract-treated T24 cells with 19 up-regulated and 1 down-regulated proteins. These de-regulated proteins were involved in apoptosis, cytoskeleton regulation, cell proliferation, proteasome activity and aerobic glycolysis. Further studies on signaling pathway demonstrated that ethanol extract treatment might inhibit urinary bladder urothelial carcinoma cell proliferation through restriction of PTEN/AKT/mTORC1 pathway via profilin 1 up-regulation. It also might evoke cell apoptosis through Diablo over-expression. The results of this study provide a global picture to further investigate the anticancer molecular mechanism of pomegranate fruit.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 22%
Student > Bachelor 1 11%
Student > Doctoral Student 1 11%
Other 1 11%
Student > Ph. D. Student 1 11%
Other 2 22%
Unknown 1 11%
Readers by discipline Count As %
Medicine and Dentistry 2 22%
Agricultural and Biological Sciences 2 22%
Business, Management and Accounting 1 11%
Pharmacology, Toxicology and Pharmaceutical Science 1 11%
Social Sciences 1 11%
Other 1 11%
Unknown 1 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 September 2016.
All research outputs
#7,267,999
of 8,409,255 outputs
Outputs from BMC Complementary and Alternative Medicine
#1,794
of 2,126 outputs
Outputs of similar age
#209,615
of 253,631 outputs
Outputs of similar age from BMC Complementary and Alternative Medicine
#51
of 73 outputs
Altmetric has tracked 8,409,255 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,126 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.6. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 73 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.