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Attention Score in Context
| Title |
Plasma exosome profiles from dairy cows with divergent fertility phenotypes
|
|---|---|
| Published in |
Journal of Dairy Science, June 2016
|
| DOI | 10.3168/jds.2016-11060 |
| Pubmed ID | |
| Authors |
M.D. Mitchell, K. Scholz-Romero, S. Reed, H.N. Peiris, Y.Q. Koh, S. Meier, C.G. Walker, C.R. Burke, J.R. Roche, G. Rice, C. Salomon |
| Abstract |
Cell-to-cell communication in physiological and pathological conditions may be influenced by neighboring cells, distant tissues, or local environmental factors. Exosomes are specific subsets of extracellular vesicles that internalize and deliver their content to near and distant sites. Exosomes may play a role in the maternal-embryo crosstalk vital for the recognition and maintenance of a pregnancy; however, their role in dairy cow reproduction has not been established. This study aimed to characterize the exosome profile in the plasma of 2 strains of dairy cow with divergent fertility phenotypes. Plasma was obtained and characterized on the basis of genetic ancestry as fertile (FERT; <23% North American genetics, New Zealand Holstein-Friesian strain, n = 8) or subfertile (SUBFERT; >92% North American genetics, North American Holstein-Friesian strain, n = 8). Exosomes were isolated by differential and buoyant density centrifugation and characterized by size distribution (nanoparticle tracking analysis, NanoSight NS500, NanoSight Ltd., Amesbury, UK), the presence of CD63 (Western blot), and their morphology (electron microscopy). The total number of exosomes was determined by quantifying the immunoreactive CD63 (ExoELISA kit, System Biosciences), and the protein content established by mass spectrometry. Enriched exosome fractions were identified as cup-shape vesicles with diameters around 100 nm and positive for the CD63 marker. The concentration of exosomes was 50% greater in FERT cows. Mass spectrometry identified 104 and 117 proteins in FERT and SUBFERT cows, of which 23 and 36 were unique, respectively. Gene ontology analysis revealed enrichment for proteins involved in immunomodulatory processes and cell-to-cell communication. Although the role of exosomes in dairy cow reproduction remains to be elucidated, their quantification and content in models with divergent fertility phenotypes could provide novel information to support both physiological and genetic approaches to improving dairy cow fertility. |
Mendeley readers
The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Croatia | 1 | 2% |
| Unknown | 59 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 9 | 15% |
| Researcher | 8 | 13% |
| Student > Ph. D. Student | 7 | 12% |
| Student > Doctoral Student | 5 | 8% |
| Student > Bachelor | 5 | 8% |
| Other | 11 | 18% |
| Unknown | 15 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 22% |
| Biochemistry, Genetics and Molecular Biology | 11 | 18% |
| Medicine and Dentistry | 7 | 12% |
| Engineering | 5 | 8% |
| Veterinary Science and Veterinary Medicine | 4 | 7% |
| Other | 3 | 5% |
| Unknown | 17 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 21. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 September 2016.
All research outputs
#1,735,128
of 25,371,288 outputs
Outputs from Journal of Dairy Science
#280
of 11,136 outputs
Outputs of similar age
#31,168
of 367,282 outputs
Outputs of similar age from Journal of Dairy Science
#8
of 168 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 11,136 research outputs from this source. They receive a mean Attention Score of 4.5. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 367,282 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 168 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.