Title |
Preparation and in vitro and in vivo characterization of cyclosporin A-loaded, PEGylated chitosan-modified, lipid-based nanoparticles
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Published in |
International Journal of Nanomedicine, February 2013
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DOI | 10.2147/ijn.s39685 |
Pubmed ID | |
Authors |
Ling Zhang, Zhi-Liang Zhao, Xiao-Hong Wei, Jin-Hua Liu |
Abstract |
A new cyclosporin A-loaded, PEGylated chitosan-modified lipid-based nanoparticle was developed to improve upon the formulation of cyclosporin A. PEGylated chitosan, synthesized in three steps using mild reaction conditions, was used to modify the nanoparticles. Cyclosporin A-loaded, PEGylated chitosan-modified nanoparticles were prepared using an emulsification/solvent evaporation method. The drug content and encapsulation efficiency of the cyclosporin A-loaded, PEGylated chitosan-modified nanoparticles were measured by high-performance liquid chromatography. The average size of the nanoparticles was determined by transmission electron microscopy and dynamic light scattering. The pharmacokinetic behavior of the nanoparticles was investigated in rabbits after intravenous injection. Cyclosporin A concentrations in a whole blood sample were analyzed by high-performance liquid chromatography using tamoxifen as the internal standard. The pharmacokinetic parameters were calculated using the 3p87 software program. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 37 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 12 | 32% |
Student > Master | 6 | 16% |
Researcher | 4 | 11% |
Student > Doctoral Student | 3 | 8% |
Professor > Associate Professor | 3 | 8% |
Other | 3 | 8% |
Unknown | 6 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 8 | 22% |
Agricultural and Biological Sciences | 6 | 16% |
Medicine and Dentistry | 4 | 11% |
Chemistry | 3 | 8% |
Engineering | 2 | 5% |
Other | 5 | 14% |
Unknown | 9 | 24% |