| Chapter title |
Enhancer RNAs
|
|---|---|
| Chapter number | 9 |
| Book title |
Enhancer RNAs
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-4035-6_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-4033-2, 978-1-4939-4035-6
|
| Authors |
Gardini, Alessandro, Alessandro Gardini Ph.D., Alessandro Gardini |
| Editors |
Ulf Andersson Ørom |
| Abstract |
Transcription occurring at gene loci results in accumulation of mature RNA molecules (i.e., mRNAs) that can be easily assayed by RT-PCR or RNA sequencing. However, the steady-state level of RNA does not accurately mirror transcriptional activity per se. In fact, RNA stability plays a major role in determining the relative abundance of any given RNA molecule. Here, I describe a protocol of Nuclear Run-On assay coupled to deep sequencing to assess real-time transcription from engaged RNA polymerase. Mapping nascent transcripts at the genome-wide scale provides a reliable measure of transcriptional activity in mammalian cells and delivers a high-resolution map of coding and noncoding transcripts that is especially useful for annotation and quantification of short-lived RNA molecules. |
X Demographics
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|---|---|---|
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| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
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|---|---|---|
| Unknown | 112 | 100% |
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|---|---|---|
| Student > Ph. D. Student | 34 | 30% |
| Student > Bachelor | 13 | 12% |
| Student > Master | 12 | 11% |
| Researcher | 11 | 10% |
| Student > Doctoral Student | 8 | 7% |
| Other | 11 | 10% |
| Unknown | 23 | 21% |
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| Immunology and Microbiology | 4 | 4% |
| Computer Science | 2 | 2% |
| Other | 4 | 4% |
| Unknown | 26 | 23% |