Title |
Eukaryotic association module in phage WO genomes from Wolbachia
|
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Published in |
Nature Communications, October 2016
|
DOI | 10.1038/ncomms13155 |
Pubmed ID | |
Authors |
Sarah R. Bordenstein, Seth R. Bordenstein |
Abstract |
Viruses are trifurcated into eukaryotic, archaeal and bacterial categories. This domain-specific ecology underscores why eukaryotic viruses typically co-opt eukaryotic genes and bacteriophages commonly harbour bacterial genes. However, the presence of bacteriophages in obligate intracellular bacteria of eukaryotes may promote DNA transfers between eukaryotes and bacteriophages. Here we report a metagenomic analysis of purified bacteriophage WO particles of Wolbachia and uncover a eukaryotic association module in the complete WO genome. It harbours predicted domains, such as the black widow latrotoxin C-terminal domain, that are uninterrupted in bacteriophage genomes, enriched with eukaryotic protease cleavage sites and combined with additional domains to forge one of the largest bacteriophage genes to date (14,256 bp). To the best of our knowledge, these eukaryotic-like domains have never before been reported in packaged bacteriophages and their phylogeny, distribution and sequence diversity imply lateral transfers between bacteriophage/prophage and animal genomes. Finally, the WO genome sequences and identification of attachment sites will potentially advance genetic manipulation of Wolbachia. |
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United Kingdom | 14 | 13% |
Brazil | 4 | 4% |
Germany | 3 | 3% |
Canada | 3 | 3% |
Netherlands | 2 | 2% |
Philippines | 2 | 2% |
India | 2 | 2% |
Spain | 2 | 2% |
Other | 12 | 11% |
Unknown | 46 | 43% |
Demographic breakdown
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Members of the public | 55 | 52% |
Scientists | 45 | 42% |
Practitioners (doctors, other healthcare professionals) | 4 | 4% |
Science communicators (journalists, bloggers, editors) | 2 | 2% |
Mendeley readers
Geographical breakdown
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United States | 7 | 2% |
Brazil | 2 | <1% |
Austria | 1 | <1% |
Germany | 1 | <1% |
Portugal | 1 | <1% |
United Kingdom | 1 | <1% |
Czechia | 1 | <1% |
Japan | 1 | <1% |
Mexico | 1 | <1% |
Other | 0 | 0% |
Unknown | 284 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 83 | 28% |
Researcher | 60 | 20% |
Student > Master | 37 | 12% |
Student > Bachelor | 30 | 10% |
Student > Doctoral Student | 12 | 4% |
Other | 39 | 13% |
Unknown | 39 | 13% |
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Biochemistry, Genetics and Molecular Biology | 75 | 25% |
Immunology and Microbiology | 18 | 6% |
Environmental Science | 6 | 2% |
Mathematics | 5 | 2% |
Other | 32 | 11% |
Unknown | 51 | 17% |