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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Transgenic Fluorescent Plasmodium cynomolgi Liver Stages Enable Live Imaging and Purification of Malaria Hypnozoite-Forms
|
|---|---|
| Published in |
PLOS ONE, January 2013
|
| DOI | 10.1371/journal.pone.0054888 |
| Pubmed ID | |
| Authors |
Annemarie Voorberg-van der Wel, Anne-Marie Zeeman, Sandra M. van Amsterdam, Alexander van den Berg, Els J. Klooster, Shiroh Iwanaga, Chris J. Janse, Geert-Jan van Gemert, Robert Sauerwein, Niels Beenhakker, Gerrit Koopman, Alan W. Thomas, Clemens H. M. Kocken |
| Abstract |
A major challenge for strategies to combat the human malaria parasite Plasmodium vivax is the presence of hypnozoites in the liver. These dormant forms can cause renewed clinical disease after reactivation through unknown mechanisms. The closely related non-human primate malaria P. cynomolgi is a frequently used model for studying hypnozoite-induced relapses. Here we report the generation of the first transgenic P. cynomolgi parasites that stably express fluorescent markers in liver stages by transfection with novel DNA-constructs containing a P. cynomolgi centromere. Analysis of fluorescent liver stages in culture identified, in addition to developing liver-schizonts, uninucleate persisting parasites that were atovaquone resistant but primaquine sensitive, features associated with hypnozoites. We demonstrate that these hypnozoite-forms could be isolated by fluorescence-activated cell sorting. The fluorescently-tagged parasites in combination with FACS-purification open new avenues for a wide range of studies for analysing hypnozoite biology and reactivation. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 33% |
| United Kingdom | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 70 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 1% |
| Indonesia | 1 | 1% |
| Brazil | 1 | 1% |
| India | 1 | 1% |
| United Kingdom | 1 | 1% |
| Unknown | 65 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 25 | 36% |
| Student > Ph. D. Student | 13 | 19% |
| Other | 4 | 6% |
| Lecturer | 4 | 6% |
| Student > Master | 4 | 6% |
| Other | 8 | 11% |
| Unknown | 12 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 26 | 37% |
| Biochemistry, Genetics and Molecular Biology | 9 | 13% |
| Medicine and Dentistry | 9 | 13% |
| Immunology and Microbiology | 7 | 10% |
| Social Sciences | 2 | 3% |
| Other | 4 | 6% |
| Unknown | 13 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 February 2016.
All research outputs
#12,870,383
of 22,699,621 outputs
Outputs from PLOS ONE
#100,293
of 193,796 outputs
Outputs of similar age
#151,286
of 280,587 outputs
Outputs of similar age from PLOS ONE
#2,436
of 5,005 outputs
Altmetric has tracked 22,699,621 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 193,796 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.0. This one is in the 47th percentile – i.e., 47% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,587 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 5,005 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.