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Mendeley readers
Attention Score in Context
| Title |
Effect of Heavy Atoms on the Thermal Stability of α-Amylase from Aspergillus oryzae
|
|---|---|
| Published in |
PLOS ONE, February 2013
|
| DOI | 10.1371/journal.pone.0057432 |
| Pubmed ID | |
| Authors |
Michihiro Sugahara, Michiyo Takehira, Katsuhide Yutani |
| Abstract |
Currently, there are no versatile and established methods for improving stability of proteins. In an entirely different approach from conventional techniques such as mutagenesis, we attempted to enhance enzyme stability of α-amylase from Aspergillus oryzae using a heavy-atom derivatization technique. We evaluated changes in stability using differential scanning calorimetry (DSC). Candidate heavy atoms were identified using the Heavy-Atom Database System HATODAS, a Web-based tool designed to assist in heavy-atom derivatization of proteins for X-ray crystallography. The denaturation temperature of α-amylase derivatized with gadolinium (Gd) or samarium (Sm) ions increased by 6.2 or 5.7°C, respectively, compared to that of the native protein (60.6°C). The binding of six Gd ions was confirmed by X-ray crystallography of the enzyme at 1.5 Å resolution. DSC and dynamic light-scattering data revealed a correlation between stability and the aggregation state upon addition of Gd ions. These results show that HATODAS search is an effective tool for selecting heavy atoms for stabilization of this protein. |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 4% |
| Unknown | 23 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 21% |
| Student > Bachelor | 4 | 17% |
| Researcher | 4 | 17% |
| Student > Ph. D. Student | 2 | 8% |
| Student > Doctoral Student | 2 | 8% |
| Other | 2 | 8% |
| Unknown | 5 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 29% |
| Biochemistry, Genetics and Molecular Biology | 6 | 25% |
| Chemistry | 2 | 8% |
| Immunology and Microbiology | 1 | 4% |
| Earth and Planetary Sciences | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 6 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 February 2013.
All research outputs
#20,184,694
of 22,699,621 outputs
Outputs from PLOS ONE
#172,949
of 193,796 outputs
Outputs of similar age
#169,822
of 193,194 outputs
Outputs of similar age from PLOS ONE
#4,405
of 5,363 outputs
Altmetric has tracked 22,699,621 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 193,796 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 193,194 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 5,363 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.