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In vitro characterization of a chitosan skin regenerating template as a scaffold for cells cultivation

Overview of attention for article published in SpringerPlus, March 2013
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Mentioned by

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2 Facebook pages

Citations

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47 Dimensions

Readers on

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67 Mendeley
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Title
In vitro characterization of a chitosan skin regenerating template as a scaffold for cells cultivation
Published in
SpringerPlus, March 2013
DOI 10.1186/2193-1801-2-79
Pubmed ID
Authors

Abu Bakar Mohd Hilmi, Ahmad Sukari Halim, Asma Hassan, Chin Keong Lim, Kartini Noorsal, Ismail Zainol

Abstract

Chitosan is a marine-derived product that has been widely used in clinical applications, especially in skin reconstruction. The mammalian scaffolds derived from bovine and porcine material have many limitations, for example, prion transmission and religious concerns. Therefore, we created a chitosan skin regenerating template (SRT) and investigated the behavior of fibroblast cell-scaffold constructs. Primary human dermal fibroblasts (HDF) were isolated and then characterized using vimentin and versican. HDF were seeded into chitosan SRT at a density of 3×10(6) cells/cm(2) for fourteen days. Histological analysis and live cells imaging revealed that the cell-chitosan constructs within interconnected porous chitosan showed significant interaction between the cells as well as between the cells and the chitosan. Scanning electron microscopy (SEM) analysis revealed cells spreading and covering the pores. As the pore sizes of the chitosan SRT range between 40-140 μm, an average porosity is about 93 ± 12.57% and water uptake ratio of chitosan SRT is 536.02 ± 14.29%, it is a supportive template for fibroblast attachment and has potential in applications as a dermal substitute.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 2 3%
Japan 1 1%
Russia 1 1%
Unknown 63 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 28%
Student > Master 13 19%
Researcher 5 7%
Student > Postgraduate 4 6%
Other 4 6%
Other 10 15%
Unknown 12 18%
Readers by discipline Count As %
Materials Science 7 10%
Medicine and Dentistry 7 10%
Agricultural and Biological Sciences 6 9%
Biochemistry, Genetics and Molecular Biology 6 9%
Chemistry 6 9%
Other 14 21%
Unknown 21 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 April 2013.
All research outputs
#18,331,227
of 22,699,621 outputs
Outputs from SpringerPlus
#1,260
of 1,852 outputs
Outputs of similar age
#148,262
of 194,736 outputs
Outputs of similar age from SpringerPlus
#57
of 126 outputs
Altmetric has tracked 22,699,621 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,852 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 21st percentile – i.e., 21% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 194,736 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 126 others from the same source and published within six weeks on either side of this one. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.