Title |
The Impact of Silver Nanoparticles Produced by Bacillus pumilus As Antimicrobial and Nematicide
|
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Published in |
Frontiers in Microbiology, November 2016
|
DOI | 10.3389/fmicb.2016.01746 |
Pubmed ID | |
Authors |
Wael M. Mahmoud, Tamer S. Abdelmoneim, Ahmed M. Elazzazy |
Abstract |
This study evaluates the potential application of silver nanoparticles (AgNPs) as antimicrobial or nematicidal agents produced by the extremophile Bacillus pumilus, which was isolated from the alkaline Wadi El-Natrun Lake in Egypt. The AgNPs were characterized by ultraviolet-visible absorption spectroscopy, transmission electron microscopy, and energy dispersive x-ray spectroscopy. The size of AgNPs formed ranged from 20.12 to 29.48 nm. Panagrellus redivivus was exposed to different concentrations (0, 50, 100, 150, and 200 μg/mL) of AgNPs in a 5 mL nematode suspension (1 × 10(3) mL(-1)). The best result occurred at AgNP concentrations of 150 and 200 μg/mL, with death rates of 80 and 91%, respectively, following 48 h of exposure. AgNPs also exhibited potent antimicrobial properties when using Gram-negative and Gram-positive human pathogens, with MIC and MBC values of 5 and 10 μg/mL, respectively. These laboratory assays prove that biologically synthesized AgNPs are an ecofriendly material that can be used in lieu of solvents or toxic chemicals. |
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Unknown | 3 | 75% |
Demographic breakdown
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Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
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Researcher | 6 | 11% |
Student > Master | 5 | 9% |
Student > Bachelor | 4 | 7% |
Student > Doctoral Student | 2 | 4% |
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Immunology and Microbiology | 2 | 4% |
Other | 7 | 13% |
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