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Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging

Overview of attention for article published in Experimental Gerontology, October 2016
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Title
Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging
Published in
Experimental Gerontology, October 2016
DOI 10.1016/j.exger.2016.10.005
Pubmed ID
Authors

Stanislava Stanojević, Ivana Ćuruvija, Veljko Blagojević, Raisa Petrović, Vesna Vujić, Mirjana Dimitrijević

Abstract

Rats of Albino Oxford (AO) strain in our animal facility exhibit a longer average healthy life span than rats of Dark Agouit (DA) strain. Since chronic activation of macrophages contributes to chronic low level inflammation common in older age, elucidation of the changes in middle-aged rats could be useful in prevention of unbalanced inflammatory response in advanced age. We have analysed the phenotype of unelicited and thioglycollate-elicited peritoneal macrophages from young and middle-aged DA and AO rats and tested functions of these cells following stimulation with lipopolysaccharide (LPS) in vitro. Unelicited cells from middle-aged DA rats produced higher amounts of proinflammatory mediators interleukin-6 (IL-6) and nitric oxide (NO), but have a diminished response to LPS stimulation then cells from young rats, in spite of increased frequency of TLR4- and CD14-expressing mature macrophages. Injection of thioglycollate robustly increased overall cytokine production in young rats' macrophages, while diminishing their response to LPS stimulation. In middle-aged DA rats injection of thioglycollate diminished IL-6 production, but increased it in response to LPS stimulation. Quite the contrary to DA rats, the macrophages from middle-aged AO rats have released diminished levels of TNF-α and NO, whereas urea production was strongly increased, when compared to the macrophages from young rats. Although the thioglycollate injection has increased the proportion of CD86(+)MHCII(+) mature macrophages in young rats, and percentages of activated TLR4(+) macrophages in both age groups of AO rats, it has not affected the cytokine production in young rats' macrophages, and the TNF-α production in middle-aged rats' macrophages. Moreover, the injection of thioglycollate has robustly increased the production of urea in macrophages derived from both age groups of AO rats. Although middle-aged rats of both strains were healthy during experiment, differences between the inflammatory responses of peritoneal macrophages of middle-aged rats of these strains might be one of the contributing factors defining their health in their advanced age. Development of strategies for the prevention of undesirable inflammatory changes in the elderly would benefit from the prospective study of the middle-aged.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Serbia 1 7%
Unknown 13 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 21%
Student > Bachelor 2 14%
Other 1 7%
Librarian 1 7%
Student > Master 1 7%
Other 0 0%
Unknown 6 43%
Readers by discipline Count As %
Medicine and Dentistry 3 21%
Environmental Science 1 7%
Immunology and Microbiology 1 7%
Agricultural and Biological Sciences 1 7%
Social Sciences 1 7%
Other 1 7%
Unknown 6 43%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 October 2016.
All research outputs
#17,285,933
of 25,380,192 outputs
Outputs from Experimental Gerontology
#2,084
of 2,798 outputs
Outputs of similar age
#210,532
of 326,284 outputs
Outputs of similar age from Experimental Gerontology
#33
of 38 outputs
Altmetric has tracked 25,380,192 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,798 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.3. This one is in the 18th percentile – i.e., 18% of its peers scored the same or lower than it.
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