Chapter title |
GC-Rich DNA Fragments and Oxidized Cell-Free DNA Have Different Effects on NF-kB and NRF2 Signaling in MSC.
|
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Chapter number | 21 |
Book title |
Circulating Nucleic Acids in Serum and Plasma – CNAPS IX
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-3-319-42044-8_21 |
Pubmed ID | |
Book ISBNs |
978-3-31-942042-4, 978-3-31-942044-8
|
Authors |
Vasilina A. Sergeeva, Svetlana V. Kostyuk, Elizaveta S. Ershova, Elena M. Malinovskaya, Tatiana D. Smirnova, Larisa V. Kameneva, Natalia N. Veiko |
Editors |
Peter B. Gahan, Michael Fleischhacker, Bernd Schmidt |
Abstract |
It has been established that cell-free DNA circulating in the bloodstream affects cells. The characteristics of cfDNA depend on the physiological state of the organism. As we showed previously, diseases can cause either GC-enrichment of the cell-free DNA pool or its oxidation. Thus, in cases of cerebral atherosclerosis, heart attack and rheumatic arthritis the cell-free DNA pool is GC-enriched and, in the case of cancer, both GC-enriched and oxidized. Herein we investigated the time-dependent effect of oxidized and GC-rich cell-free DNA on NF-kB and NRF2 signaling pathways in human mesenchymal stem cells and showed that they affect cells in different ways. Oxidized DNA drastically increases expression of NRF2 in a short period of time, but the effect does not last long. GC-rich DNA causes a prolonged increase in mRNA levels of NF-kB and NRF2 which lasts 48 and 24 h, respectively. |
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