Title |
Effect of substrate stiffness on early human embryonic stem cell differentiation
|
---|---|
Published in |
Journal of Biological Engineering, March 2013
|
DOI | 10.1186/1754-1611-7-7 |
Pubmed ID | |
Authors |
Nikolai Eroshenko, Rukmani Ramachandran, Vamsi K Yadavalli, Raj R Rao |
Abstract |
The pluripotency and self renewing properties of human embryonic stem cells (hESC) make them a valuable tool in the fields of developmental biology, pharmacology and regenerative medicine. Therefore, there exists immense interest in devising strategies for hESC propagation and differentiation. Methods involving simulation of the native stem cell microenvironment, both chemical and physical, have received a lot of attention in recent years. Equally important is evidence that cells can also sense the mechanical properties of their microenvironment. In this study, we test the hypothesis that hESCs accept mechanical cues for differentiation from the substrate by culturing them on flexible polydimethylsiloxane (PDMS) of varying stiffness. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 2 | 1% |
Switzerland | 1 | <1% |
Italy | 1 | <1% |
France | 1 | <1% |
Denmark | 1 | <1% |
United States | 1 | <1% |
Unknown | 167 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 55 | 32% |
Researcher | 22 | 13% |
Student > Bachelor | 21 | 12% |
Student > Master | 16 | 9% |
Student > Doctoral Student | 12 | 7% |
Other | 18 | 10% |
Unknown | 30 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 40 | 23% |
Engineering | 35 | 20% |
Biochemistry, Genetics and Molecular Biology | 22 | 13% |
Medicine and Dentistry | 9 | 5% |
Physics and Astronomy | 8 | 5% |
Other | 28 | 16% |
Unknown | 32 | 18% |