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Combined small angle X-ray solution scattering with atomic force microscopy for characterizing radiation damage on biological macromolecules

Overview of attention for article published in BMC Structural Biology, October 2016
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Title
Combined small angle X-ray solution scattering with atomic force microscopy for characterizing radiation damage on biological macromolecules
Published in
BMC Structural Biology, October 2016
DOI 10.1186/s12900-016-0068-2
Pubmed ID
Authors

Luca Costa, Alexander Andriatis, Martha Brennich, Jean-Marie Teulon, Shu-wen W. Chen, Jean-Luc Pellequer, Adam Round

Abstract

Synchrotron radiation facilities are pillars of modern structural biology. Small-Angle X-ray scattering performed at synchrotron sources is often used to characterize the shape of biological macromolecules. A major challenge with high-energy X-ray beam on such macromolecules is the perturbation of sample due to radiation damage. By employing atomic force microscopy, another common technique to determine the shape of biological macromolecules when deposited on flat substrates, we present a protocol to evaluate and characterize consequences of radiation damage. It requires the acquisition of images of irradiated samples at the single molecule level in a timely manner while using minimal amounts of protein. The protocol has been tested on two different molecular systems: a large globular tetremeric enzyme (β-Amylase) and a rod-shape plant virus (tobacco mosaic virus). Radiation damage on the globular enzyme leads to an apparent increase in molecular sizes whereas the effect on the long virus is a breakage into smaller pieces resulting in a decrease of the average long-axis radius. These results show that radiation damage can appear in different forms and strongly support the need to check the effect of radiation damage at synchrotron sources using the presented protocol.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 50%
Student > Bachelor 2 25%
Student > Ph. D. Student 1 13%
Researcher 1 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 38%
Engineering 2 25%
Physics and Astronomy 1 13%
Chemistry 1 13%
Biochemistry, Genetics and Molecular Biology 1 13%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 November 2016.
All research outputs
#11,990,497
of 13,526,991 outputs
Outputs from BMC Structural Biology
#155
of 181 outputs
Outputs of similar age
#241,975
of 288,789 outputs
Outputs of similar age from BMC Structural Biology
#10
of 12 outputs
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