@wildthang1276 @OrsiWilliam @mSystemsJ The low abundance OTUs/ASVs in our negative controls were typically found in higher abundance in other samples that were on the sequencing run, so I suspect they are mostly from barcode crosstalk during sequencing. @d
Interesting and very useful read on how to mitigate index hopping in Illumina data https://t.co/DolxfoaKBD #indexhopping #illumina #ngs
Nice to see Illumina finally addressing this issue! Quality filtering index reads also considerably helps: https://t.co/2VV64ixx39 https://t.co/isxcSzobmB
RT @quendi: Quality filtering of Illumina index reads mitigates sample cross-talk https://t.co/ygG5tcPVGZ